Evidence map›Paper›PMID 34301168›Full record

ArticleJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc2021

RT-rtPCR quantification of circulating microRNAs in plasma and serum samples from healthy domestic cats.

Marcela C Scalon, Christine S Martins, Gabriel G Ferreira, Franciele Schlemmer, Ricardo Titze-de-Almeida, Giane R Paludo

Abstract read
In one paragraph

Article in Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Marcela C ScalonVeterinary Clinical Pathology Laboratory, College of Agronomy and Veterinary Medicine, University of Brasilia, Brasilia, Brazil.ORCID https://orcid.org/0000-0002-3686-8413
Christine S MartinsVeterinary Clinical Pathology Laboratory, College of Agronomy and Veterinary Medicine, University of Brasilia, Brasilia, Brazil.
Gabriel G FerreiraTechnology for Gene Therapy Laboratory, College of Agronomy and Veterinary Medicine, University of Brasilia, Brasilia, Brazil.
Franciele SchlemmerTechnology for Gene Therapy Laboratory, College of Agronomy and Veterinary Medicine, University of Brasilia, Brasilia, Brazil.
Ricardo Titze-de-AlmeidaTechnology for Gene Therapy Laboratory, College of Agronomy and Veterinary Medicine, University of Brasilia, Brasilia, Brazil.
Giane R PaludoVeterinary Clinical Pathology Laboratory, College of Agronomy and Veterinary Medicine, University of Brasilia, Brasilia, Brazil.ORCID https://orcid.org/0000-0002-9112-1718

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at a post-transcriptional level by silencing targeted messenger RNA (mRNA). Most studies concerning miRNA expression use solid tissue samples. However, circulating miRNAs from different body fluids have recently emerged as diagnostic and prognostic molecules, given that they hold informative value and have increased stability in cell-free form. Blood sampling of cats can be challenging given their small body size and because they often experience distress when handled. We quantified miR-20a, -192, -365, -15b-5p, and -16-5p from plasma and serum samples of 10 healthy domestic cats. Our RT-rtPCR procedure used 100 µL of either plasma or serum samples as sources of biomarker molecules. However, serum provided higher amounts of miRNA than plasma samples, with a

Indexed as

Circulating MicroRNAMicroRNAsAnimalsBiomarkersCatsPrognosisReverse Transcriptase Polymerase Chain ReactionBiomarkersCirculating MicroRNAMicroRNAsblooddiagnosisfeline diseasesmicroRNA

Identifiers

PMID34301168
PMCPMC8546485

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.