ArticleInternational journal of molecular sciences2021
Evaluation of MC3T3-E1 Cell Osteogenesis in Different Cell Culture Media.
Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
40 citing papers in PubMed, 63 citations in OpenAlex.
- Osteogenic, antiresorptive, and toxicological evaluation of alpha-mangostin in MC3T3-E1 cells and medaka fish.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2026Article
- Synthesis and Properties of SPI/PLA-PCL Composite Microspheres.Micromachines · 2026Article
- Modulation of Intestinal-Bone Crosstalk by a Standardised Nutraceutical Combination: An In Vitro Mechanistic Study.Nutrients · 2026Article
- Advanced models of lobular breast cancer metastasis capture clinical organ tropism, endocrine response, and bone remodeling.bioRxiv : the preprint server for biology · 2026Article
- Comparative Biocompatibility of Portland-Based Endodontic Cements Containing Niobium and/or Bismuth Oxides in Rat Subcutaneous Tissue.BioMed research international · 2026Article
- A cellular automaton model of osteogenic differentiation reveals identifiability limits of endpoint assays.Frontiers in systems biology · 2026Article
- Article
- A newly designed flexible hydrated-hardening bone graft (FHBG) promotes bone regeneration and in vivo calvarial repair.Biomedical journal · 2025Article
- Magnesium Ion-Mediated Regulation of Osteogenesis and Osteoclastogenesis in 2D Culture and 3D Collagen/Nano-Hydroxyapatite Scaffolds for Enhanced Bone Repair.Journal of functional biomaterials · 2025Article
- Osmotic stress inhibits osteoblast differentiation and mineralization by suppressing TRPV4-mediated calcium influx.Journal of bone and mineral metabolism · 2025Article
- Effect of vacancies on blue-colored calcium phosphate scaffolds derived from Nile tilapia bone powder.Scientific reports · 2025Article
- Antioxidant Intervention Against Microplastic Hazards.Antioxidants (Basel, Switzerland) · 2025Review
- Synergistic Crosstalk of PACAP and Notch Signaling Pathways in Bone Development.International journal of molecular sciences · 2025Article
- Genetic Profiling of MC3T3-E1 Cells in Different Media: Implications for In Vitro Screening Development.Biomedicines · 2025Article
- In Vitro Engineered ECM-incorporated Hydrogels for Osteochondral Tissue Repair: A Cell-Free Approach.Advanced healthcare materials · 2025Article
- Potential of Liposomal FTY720 for Bone Regeneration: Proliferative, Osteoinductive, Chemoattractive, and Angiogenic Properties Compared to Free Bioactive Lipid.International journal of nanomedicine · 2025Article
- Evaluating the potential of graphene oxide to promote skeletal muscle complex regeneration.Frontiers in bioengineering and biotechnology · 2025Article
- Pro-differentiative, Pro-adhesive and Pro-migratory Activities of Isorhamnetin in MC3T3-E1 Osteoblasts via Activation of ERK-dependent BMP2-Smad Signaling.Cell biochemistry and biophysics · 2024Article
- Differential expression of osteoblast-like cells on self-organized titanium dioxide nanotubes.Journal of dental sciences · 2024Article
- Functional mechanism and clinical implications of mir-1271-5p in pilon fracture healing processes.Journal of orthopaedic surgery and research · 2024Article
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
Many biomaterials have been evaluated using cultured cells. In particular, osteoblast-like cells are often used to evaluate the osteocompatibility, hard-tissue-regeneration, osteoconductive, and osteoinductive characteristics of biomaterials. However, the evaluation of biomaterial osteogenesis-inducing capacity using osteoblast-like cells is not standardized; instead, it is performed under laboratory-specific culture conditions with different culture media. However, the effect of different media conditions on bone formation has not been investigated. Here, we aimed to evaluate the osteogenesis of MC3T3-E1 cells, one of the most commonly used osteoblast-like cell lines for osteogenesis evaluation, and assayed cell proliferation, alkaline phosphatase activity, expression of osteoblast markers, and calcification under varying culture media conditions. Furthermore, the various media conditions were tested in uncoated plates and plates coated with collagen type I and poly-L-lysine, highly biocompatible molecules commonly used as pseudobiomaterials. We found that the type of base medium, the presence or absence of vitamin C, and the freshness of the medium may affect biomaterial regeneration. We posit that an in vitro model that recapitulates in vivo bone formation should be established before evaluating biomaterials.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.