ArticleInternational journal of molecular sciences2021
Placental Villous Explant Culture 2.0: Flow Culture Allows Studies Closer to the In Vivo Situation.
Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Advantages, limitations, and innovative considerations for established and emerging models of human placental syncytiotrophoblast.Human reproduction update · 2026Review
- Rethinking preeclampsia: six paradigm shifts in placental pathophysiology for maternal-foetal medicine.Frontiers in medicine · 2026Review
- Monkeypox Virus Subverts the Inflammatory Response of Macrophages at the Maternal-Fetal Interface.Journal of medical virology · 2025Article
- The Interplay of Molecular Factors and Morphology in Human Placental Development and Implantation.Biomedicines · 2024Review
- TGFβ signalling: a nexus between inflammation, placental health and preeclampsia throughout pregnancy.Human reproduction update · 2024Review
- In Vitro Culturing of Human Term Placental Explants.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Flow in fetoplacental-like microvessels in vitro enhances perfusion, barrier function, and matrix stability.Science advances · 2023Article
- Identification of optimal conditions for human placental explant culture and extracellular vesicle release.iScience · 2023Article
- A comprehensive review of human trophoblast fusion models: recent developments and challenges.Cell death discovery · 2023Review
- Fluid shear stress induces a shift from glycolytic to amino acid pathway in human trophoblasts.Cell & bioscience · 2023Article
- Generating Functional Multicellular Organoids from Human Placenta Villi.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Exploring maternal-fetal interface with in vitro placental and trophoblastic models.Frontiers in cell and developmental biology · 2023Review
- Human-Based New Approach Methodologies in Developmental Toxicity Testing: A Step Ahead from the State of the Art with a Feto-Placental Organ-on-Chip Platform.International journal of environmental research and public health · 2022Review
- Modelling human placental villous development: designing cultures that reflect anatomy.Cellular and molecular life sciences : CMLS · 2022Review
- Placental Related Disorders of Pregnancy.International journal of molecular sciences · 2022Article
- The effect of ethanol and nicotine on ER stress in human placental villous explants.Current research in toxicology · 2022Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
During pregnancy, freely floating placental villi are adapted to fluid shear stress due to placental perfusion with maternal plasma and blood. In vitro culture of placental villous explants is widely performed under static conditions, hoping the conditions may represent the in utero environment. However, static placental villous explant culture dramatically differs from the in vivo situation. Thus, we established a flow culture system for placental villous explants and compared commonly used static cultured tissue to flow cultured tissue using transmission and scanning electron microscopy, immunohistochemistry, and lactate dehydrogenase (LDH) and human chorionic gonadotropin (hCG) measurements. The data revealed a better structural and biochemical integrity of flow cultured tissue compared to static cultured tissue. Thus, this new flow system can be used to simulate the blood flow from the mother to the placenta and back in the most native-like in vitro system so far and thus can enable novel study designs.
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Registered trials
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