Evidence map›Paper›PMID 34297228›Full record

ArticleExperimental & applied acarology2021

Characterization of AV422 from Haemaphysalis flava ticks in vitro.

Lei Liu, Hao Tang, De-Yong Duan, Jin-Bao Liu, Jie Wang, Li-Li Feng, Tian-Yin Cheng

Abstract read
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In one paragraph

Article in Experimental & applied acarology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.7field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Lei LiuHunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Research Center for Parasites & Vectors, Hunan Collaborative Innovation Center for Safety Production of Livestock and Poultry, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China.
Hao TangHunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Research Center for Parasites & Vectors, Hunan Collaborative Innovation Center for Safety Production of Livestock and Poultry, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China.
De-Yong DuanHunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Research Center for Parasites & Vectors, Hunan Collaborative Innovation Center for Safety Production of Livestock and Poultry, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China.
Jin-Bao LiuHunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Research Center for Parasites & Vectors, Hunan Collaborative Innovation Center for Safety Production of Livestock and Poultry, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China.
Jie WangHunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Research Center for Parasites & Vectors, Hunan Collaborative Innovation Center for Safety Production of Livestock and Poultry, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China.
Li-Li FengHunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Research Center for Parasites & Vectors, Hunan Collaborative Innovation Center for Safety Production of Livestock and Poultry, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China.
Tian-Yin ChengHunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Research Center for Parasites & Vectors, Hunan Collaborative Innovation Center for Safety Production of Livestock and Poultry, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China. hn5368@163.com.ORCID http://orcid.org/0000-0001-8427-2908
Hunan Agricultural University · CN

Funding

the Double first-class construction project of Hunan Agricultural University SYL201802016the Key Research and Development Program of Hunan Province 2019NK2181the science fund for distinguished young scholars of changsha city, china kq2009055
6 · The paper itself

Abstract

Ticks are hematophagous ectoparasites and cause a major public health threat worldwide. Development of anti-tick vaccines is regarded to be an optimal alternative for tick control. AV422, a unique protein in ticks, is secreted into hosts during blood-feeding, but its roles are not confirmed in Haemaphysalis flava ticks. We retrieved a gene fragment encoding AV422 from a transcriptome dataset of H. flava, and based on it, we reconstructed the full length of AV422 from H. flava (Hf-AV422) by rapid amplification of cDNA ends. Expression profiles of Hf-AV422 in whole ticks and organs of different engorgement levels were determined by qPCR. Then its opening reading frame (ORF) was expressed in Escherichia coli strain BL21 (DE3). The prothrombin time (PT), activated partial thromboplastin time (APTT) and thrombin time (TT) assays were conducted to test anticoagulant activities of the purified recombinant protein (rHf-AV422). The full length of AV422 was 1152 bp. Hf-AV422 showed to be conserved as indicated by multiple sequence alignment. Expression of Hf-AV422 was significantly higher in salivary glands and cuticles than in ovaries. Its expression in whole ticks decreased during engorgement with the highest levels in 1/4 engorged ticks. rHf-AV422 prolonged PT, APTT and TT when incubated with rabbit plasma. Our data demonstrated that Hf-AV422 is a conserved salivary protein with anticoagulant activity. Further studies are needed to test in detail its functional properties to ensure it an adequate antigen candidate for the development of broad-spectrum vaccines against ticks.

Indexed as

IxodidaeTicksAnimalsDNA, ComplementaryRabbitsRecombinant ProteinsTranscriptomeDNA, ComplementaryRecombinant ProteinsAnticoagulationAntigen candidateBlood-feedingRecombinant proteinTick

Identifiers

PMID34297228
OpenAlexW3186902155

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.