Article3 Biotech2021
Development of a SYBR Green-based RT-qPCR assay for the detection of Indian citrus ringspot virus.
Article in 3 Biotech, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 16 citations in OpenAlex.
- Development of diagnostic tools and discovery of two novel Indian citrus ringspot virus species: insights into global mandarivirus phylogeography.Frontiers in microbiology · 2025Article
- Article
- Recent advances and challenges in plant viral diagnostics.Frontiers in plant science · 2024Review
- Article
- A novel study on bean common mosaic virus accumulation shows disease resistance at the initial stage of infection inFrontiers in genetics · 2023Article
- Article
- Huanglongbing Pandemic: Current Challenges and Emerging Management Strategies.Plants (Basel, Switzerland) · 2022Review
- A Comprehensive Analysis of Citrus Tristeza Variants of Bhutan and Across the World.Frontiers in microbiology · 2022Article
- Development of a real-time RT-PCR method for the detection of3 Biotech · 2021Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Indian citrus ringspot virus (ICRSV) that causes ringspot disease, especially to 'Kinnow mandarin' hampers the sustainability of crop production. Presently, the disease is not amenable for control through host resistance or the introduction of chemicals, hence raising virus-free plants is one of the most effective approaches to manage the disease. Consequently, it is necessary to develop rapid, sensitive, specific, and early diagnostic methods for disease control. In the present study, newly designed primers targeting a 164 bp region of the ICRSV coat protein gene were used to develop and optimize a SYBR Green-based quantitative reverse transcription polymerase chain reaction (RT-qPCR) assay, for the detection of ICRSV. The RT-qPCR assay was evaluated and confirmed using viral RNA extracted from ICRSV infected plants maintained in screen house as well as field samples. The standard curves displayed a dynamic linear range across eight log units of ICRSV-cRNA copy number ranging from 9.48.1 fmol (5.709 × 10
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Registered trials
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