Evidence map›Paper›PMID 34295028›Full record

ArticleJournal of parasitic diseases : official organ of the Indian Society for Parasitology2021

Performance of formalin ethyl acetate, percoll sedimentation and ELISA for the detection of

Amal Farahat Allam, Hend Aly El-Taweel, Hoda Fahmy Farag, Abdulsalam Farag Ibrahim Mohamed, Safia Saleh Khalil, Amel Youssef Shehab

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of parasitic diseases : official organ of the Indian Society for Parasitology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.5field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Amal Farahat AllamDepartment of Parasitology, Medical Research Institute, University of Alexandria, 165 El Horreya Avenue, El Hadara, Alexandria, Egypt.ORCID 0000-0002-4965-9469
Hend Aly El-TaweelDepartment of Parasitology, Medical Research Institute, University of Alexandria, 165 El Horreya Avenue, El Hadara, Alexandria, Egypt.
Hoda Fahmy FaragDepartment of Parasitology, Medical Research Institute, University of Alexandria, 165 El Horreya Avenue, El Hadara, Alexandria, Egypt.
Abdulsalam Farag Ibrahim MohamedFaculty of Medical Technology, University of Omar Al Mukhtar, Al Bayda, Libya.
Safia Saleh KhalilDepartment of Parasitology, Medical Research Institute, University of Alexandria, 165 El Horreya Avenue, El Hadara, Alexandria, Egypt.
Amel Youssef ShehabDepartment of Parasitology, Medical Research Institute, University of Alexandria, 165 El Horreya Avenue, El Hadara, Alexandria, Egypt.
Alexandria University · EGOmar Al-Mukhtar University · LY

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study aimed to assess the performance of formalin ethyl acetate (FEA)/modified Ziehl-Neelsen (MZN), and percoll technique/MZN for the diagnosis of cryptosporidiosis among asymptomatic children compared to ELISA coproantigen. The study was conducted on 100 children in a rural area in Kafr El-Sheikh governorate. Stool samples were collected and examined by the three techniques. Microscopic examination revealed the presence of acid-fast stained oocysts and non-acid fast ghost oocysts. The overall prevalence rate was 7% with an infection intensity of 1-5 oocysts/oil immersion field. FEA/MZN technique showed the highest diagnostic performance (5%) with 71.4% sensitivity and 98% negative predictive value (NPV) compared to the other techniques. ELISA revealed 3% prevalence, 42.9% sensitivity and 96% NPV. Percoll/MZN gave the lowest prevalence, sensitivity and NPV (1%, 14.29% and 93.9% respectively). Agreement fluctuated between moderate and poor regarding FEA/MZN versus ELISA and percoll/MZN versus both techniques. In conclusion, FEA/MZN gave the top diagnostic performance, yet it missed some positive cases. Its combination with ELISA coproantigen might prove beneficial for

Indexed as

CryptosporidiumELISAFormalin ethyl acetatePercoll

Identifiers

PMID34295028
PMCPMC8254688
OpenAlexW3125931926

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.