ArticleScientific reports2021
Enhanced brightness of bacterial luciferase by bioluminescence resonance energy transfer.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 35 citations in OpenAlex.
- Metabolically robust ratiometric autoluminescent reporters.Nature plants · 2026Article
- Non-invasive imaging of defence responses in plants.Nature communications · 2026Article
- Bacterial bioluminescence for the real time andAntimicrobial agents and chemotherapy · 2026Article
- Luminescence-Based Strategies for Detecting β-Lactamase Activity: A Review of the Last Decade.Life (Basel, Switzerland) · 2026Review
- Autonomous Bioluminescence Systems: From Molecular Mechanisms to Emerging Applications.JACS Au · 2025Review
- Mechanistic insights into the orthogonal functionality of an AHL-mediated quorum-sensing circuit inSynthetic and systems biotechnology · 2025Article
- Immunomodulating melatonin-decorated silica nanoparticles suppress bacterial wilt (Ralstonia solanacearum) in tomato (Solanum lycopersicum L.) through fine-tuning of oxidative signaling and rhizosphere bacterial community.Journal of nanobiotechnology · 2024Article
- Autonomous multicolor bioluminescence imaging in bacteria, mammalian, and plant hosts.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Validation of a Standard Luminescence Method for the Fast Determination of the Antimicrobial Activity of Nanoparticles inNanomaterials (Basel, Switzerland) · 2022Article
- Cryo-EM structure of the fatty acid reductase LuxC-LuxE complex provides insights into bacterial bioluminescence.The Journal of biological chemistry · 2022Article
- Strategies for Improving Small-Molecule Biosensors in Bacteria.Biosensors · 2022Review
- Bacterial bioluminescence assay for bioanalysis and bioimaging.Analytical and bioanalytical chemistry · 2022Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using the lux operon (luxCDABE) of bacterial bioluminescence system as an autonomous luminous reporter has been demonstrated in bacteria, plant and mammalian cells. However, applications of bacterial bioluminescence-based imaging have been limited because of its low brightness. Here, we engineered the bacterial luciferase (heterodimer of luxA and luxB) by fusion with Venus, a bright variant of yellow fluorescent protein, to induce bioluminescence resonance energy transfer (BRET). By using decanal as an externally added substrate, color change and ten-times enhancement of brightness was achieved in Escherichia coli when circularly permuted Venus was fused to the C-terminus of luxB. Expression of the Venus-fused luciferase in human embryonic kidney cell lines (HEK293T) or in Nicotiana benthamiana leaves together with the substrate biosynthesis-related genes (luxC, luxD and luxE) enhanced the autonomous bioluminescence. We believe the improved luciferase will forge the way towards the potential development of autobioluminescent reporter system allowing spatiotemporal imaging in live cells.
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