ArticleMicrobial cell factories2021
Effects of acuC on the growth development and spinosad biosynthesis of Saccharopolyspora spinosa.
Article in Microbial cell factories, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 12 citations in OpenAlex.
- Systematic Engineering of Proteases in Saccharopolyspora Spinosa Reveals Synergistic Enhancement of Spinosad Biosynthesis via Substrate Flux Optimization.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- CRISPRi-mediated multigene downregulating redirects the metabolic flux to spinosad biosynthesis inSynthetic and systems biotechnology · 2025Article
- Genome Combination Improvement Strategy Promotes Efficient Spinosyn Biosynthesis inJournal of agricultural and food chemistry · 2025Article
- Coordinated regulation of propionyl-CoA carboxylase subunits drives precursor flux optimization in spinosad production.Frontiers in microbiology · 2025Article
- Enhanced triacylglycerol metabolism contributes to the efficient biosynthesis of spinosad inSynthetic and systems biotechnology · 2024Article
- ARTP/NTG Compound Mutagenesis Improved the Spinosad Production and the Insecticidal Virulence ofInternational journal of molecular sciences · 2024Article
- Effects of a Pirin-like protein on strain growth and spinosad biosynthesis in Saccharopolyspora spinosa.Applied microbiology and biotechnology · 2023Article
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Authors and funding
10 authors at 1 institution in 1 country.
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Abstract
backgroundAcetoin utilization protein (acuC) is a type I histone deacetylase which is highly conserved in bacteria. The acuC gene is related to the acetylation/deacetylation posttranslational modification (PTM) system in S. spinosa. Spinosyns, the secondary metabolites produced by Saccharopolyspora spinosa, are the active ingredients in a family of insect control agents. However, the specific functions and influences of acuC protein in S. spinosa are yet to be characterized.
resultsThe knockout strain and overexpression strain were constructed separately with the shuttle vector pOJ260. The production of spinosyns A and D from S. spinosa-acuC were 105.02 mg/L and 20.63 mg/L, which were 1.82-fold and 1.63-fold higher than those of the wild-type strain (57.76 mg/L and 12.64 mg/L), respectively. The production of spinosyns A and D from S. spinosa-ΔacuC were 32.78 mg/L and 10.89 mg/L, respectively. The qRT-PCR results of three selected genes (bldD, ssgA and whiA) confirmed that the overexpression of acuC affected the capacities of mycelial differentiation and sporulation. Comparative proteomics analysis was performed on these strains to investigate the underlying mechanism leading to the enhancement of spinosad yield.
conclusionsThis study first systematically analysed the effects of overexpression acuC on the growth of S. spinosa and the production of spinosad. The results identify the differentially expressed proteins and provide evidences to understand the acetylation metabolic mechanisms which can lead to the increase of secondary metabolites.
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