ArticlePloS one2021
In vivo recellularization of xenogeneic vascular grafts decellularized with high hydrostatic pressure method in a porcine carotid arterial interpose model.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 21 citations in OpenAlex.
- Recellularization of decellularized vascular grafts via aligned seeding of endothelial cells derived from human iPS cells.Scientific reports · 2025Article
- Optimizing extracellular matrix for endothelial differentiation using a design of experiments approach.Scientific reports · 2025Article
- Surgical mechanical properties of perfused decellularized massive bone allografts: An comparative in vivo test.PloS one · 2025Article
- Effects of the matrix-bounded nanovesicles of high-hydrostatic pressure decellularized tissues on neural regeneration.Science and technology of advanced materials · 2024Article
- Decellularized vascularized bone grafts as therapeutic solution for bone reconstruction: A mechanical evaluation.PloS one · 2023Article
- Preparation and Use of Decellularized Extracellular Matrix for Tissue Engineering.Journal of functional biomaterials · 2022Review
- Extraction and Biological Evaluation of Matrix-Bound Nanovesicles (MBVs) from High-Hydrostatic Pressure-Decellularized Tissues.International journal of molecular sciences · 2022Article
- Application of decellularized vascular matrix in small-diameter vascular grafts.Frontiers in bioengineering and biotechnology · 2022Review
- Extracellular Matrix-Based Biomaterials for Cardiovascular Tissue Engineering.Journal of cardiovascular development and disease · 2021Review
- Effect of luminal surface structure of decellularized aorta on thrombus formation and cell behavior.PloS one · 2021Article
- A narrative review of vascular conduits for coronary artery bypass grafting.Cell transplantationReview
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Autologous vascular grafts are widely used in revascularization surgeries for small caliber targets. However, the availability of autologous conduits might be limited due to prior surgeries or the quality of vessels. Xenogeneic decellularized vascular grafts from animals can potentially be a substitute of autologous vascular grafts. Decellularization with high hydrostatic pressure (HHP) is reported to highly preserve extracellular matrix (ECM), creating feasible conditions for recellularization and vascular remodeling after implantation. In the present study, we conducted xenogeneic implantation of HHP-decellularized bovine vascular grafts from dorsalis pedis arteries to porcine carotid arteries and posteriorly evaluated graft patency, ECM preservation and recellularization. Avoiding damage of the luminal surface of the grafts from drying significantly during the surgical procedure increased the graft patency at 4 weeks after implantation (P = 0.0079). After the technical improvement, all grafts (N = 5) were patent with mild stenosis due to intimal hyperplasia at 4 weeks after implantation. Neither aneurysmal change nor massive thrombosis was observed, even without administration of anticoagulants nor anti-platelet agents. Elastica van Gieson and Sirius-red stainings revealed fair preservation of ECM proteins including elastin and collagen after implantation. The luminal surface of the grafts were thoroughly covered with von Willebrand factor-positive endothelium. Scanning electron microscopy of the luminal surface of implanted grafts exhibited a cobblestone-like endothelial cell layer which is similar to native vascular endothelium. Recellularization of the tunica media with alpha-smooth muscle actin-positive smooth muscle cells was partly observed. Thus, we confirmed that HHP-decellularized grafts are feasible for xenogeneic implantation accompanied by recellularization by recipient cells.
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