Evidence map›Paper›PMID 34292542›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Study of Fe-S Cluster Proteins in Methanococcus maripaludis , a Model Archaeal Organism.

Cuiping Zhao, Christina A Roberts, Ian J Drake, Yuchen Liu

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. A minimal SufBMicrobiology spectrum · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Cuiping ZhaoDepartment of Biochemistry and Microbiology, Rutgers University, New Brunswick, NJ, USA.
Christina A RobertsAstrix Technology Group, Red Bank, NJ, USA.
Ian J DrakeCorporate Strategic Research, ExxonMobil Research & Engineering Company, Annandale, NJ, USA.
Yuchen LiuCorporate Strategic Research, ExxonMobil Research & Engineering Company, Annandale, NJ, USA. yuchen.liu@exxonmobil.com.
ExxonMobil (United States) · USRutgers, The State University of New Jersey · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Iron-sulfur (Fe-S) clusters are among the oldest and most versatile cofactors present in all domains of life. Many bacterial and eukaryotic Fe-S proteins have been well-characterized, whereas the archaeal ones are less studied. Fe-S proteins are particularly abundant and play essential roles in methanogenic archaea. Methanococcus maripaludis is a model methanogen with available genetic tools. Here, we describe the techniques for anaerobic cultivation of M. maripaludis with formate, liposome-mediated transformation, expression and anoxic affinity purification of Fe-S proteins, Fe-S cluster reconstitution, and analysis of Fe-S proteins by UV-visible absorption spectroscopy.

Indexed as

MethanococcusArchaeal ProteinsIron-Sulfur ProteinsSulfurArchaeal ProteinsIron-Sulfur ProteinsSulfurAnaerobic cultivationAnoxic protein purificationArchaeaFe-S cluster reconstitutionIron-sulfur clustersLiposome-mediated transformationMethanococcusMethanogenUV-visible absorption spectroscopy

Identifiers

PMID34292542
OpenAlexW3183971105

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.