ArticleeLife2021
Pioneer neutrophils release chromatin within in vivo swarms.
Article in eLife, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed, 61 citations in OpenAlex.
- Review
- [Research progress on the role of calcitonin gene-related peptide in the repair of diabetic wounds].Zhonghua shao shang yu chuang mian xiu fu za zhi · 2026Review
- Arachidonic acid availability controls neutrophil swarm initiation and scaling.bioRxiv : the preprint server for biology · 2026Article
- Real-time characterisation of microbe-induced inflammation using a novel zebrafish larval corneal injury and infection model.Communications biology · 2026Article
- Chemical and Mechanical Regulation of Leukocyte Migration.Cold Spring Harbor perspectives in biology · 2026Review
- The Regulation of Oxidative Stress Is a Conserved Response to RNA Virus Infection in Fish.Antioxidants (Basel, Switzerland) · 2026Article
- Harnessing population genetics and animal models to uncover the genetics of skin scarring.Journal of plastic, reconstructive & aesthetic surgery : JPRAS · 2026Review
- Review
- Metainflammation alters neutrophil function and migration in vivo in response to tissue injury.Journal of leukocyte biology · 2025Article
- ATG5 suppresses type I IFN-dependent neutrophil effector functions during Mycobacterium tuberculosis infection in mice.Nature microbiology · 2025Article
- Neutrophils secrete exosome-associated DNA to resolve sterile acute inflammation.Nature cell biology · 2025Article
- Small Fish, Big Answers: Zebrafish and the Molecular Drivers of Metastasis.International journal of molecular sciences · 2025Review
- Synergistic Wound Healing: Unraveling the Multi-Target Effects of Traditional Chinese Medicine and Its Biomaterials on Chronic Wound Pathways.International journal of nanomedicine · 2025Review
- Self-extinguishing relay waves enable homeostatic control of human neutrophil swarming.Developmental cell · 2024Article
- Article
- Cellular and molecular mechanisms of skin wound healing.Nature reviews. Molecular cell biology · 2024Review
- Live Imaging of Cutaneous Wound Healing after Rotary Tool Injury in Zebrafish.The Journal of investigative dermatology · 2024Article
- Auto-amplification and spatial propagation of neutrophil extracellular traps.Communications biology · 2024Article
- Arp2/3 complex and the pentose phosphate pathway regulate late phases of neutrophil swarming.iScience · 2024Article
- Redox processes are major regulators of leukotriene synthesis in neutrophils exposed to bacteriaFrontiers in immunology · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 4 institutions in 3 countries.
Funding
Abstract
Neutrophils are rapidly recruited to inflammatory sites where their coordinated migration forms clusters, a process termed neutrophil swarming. The factors that modulate early stages of neutrophil swarming are not fully understood, requiring the development of new in vivo models. Using transgenic zebrafish larvae to study endogenous neutrophil migration in a tissue damage model, we demonstrate that neutrophil swarming is a conserved process in zebrafish immunity, sharing essential features with mammalian systems. We show that neutrophil swarms initially develop around an individual pioneer neutrophil. We observed the violent release of extracellular cytoplasmic and nuclear fragments by the pioneer and early swarming neutrophils. By combining in vitro and in vivo approaches to study essential components of neutrophil extracellular traps (NETs), we provide in-depth characterisation and high-resolution imaging of the composition and morphology of these release events. Using a photoconversion approach to track neutrophils within developing swarms, we identify that the fate of swarm-initiating pioneer neutrophils involves extracellular chromatin release and that the key NET components gasdermin, neutrophil elastase, and myeloperoxidase are required for the swarming process. Together our findings demonstrate that release of cellular components by pioneer neutrophils is an initial step in neutrophil swarming at sites of tissue injury.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.