Evidence map›Paper›PMID 34291583›Full record

ArticleEMBO molecular medicine2021

Fragmentation patterns and personalized sequencing of cell-free DNA in urine and plasma of glioma patients.

Florent Mouliere, Christopher G Smith, Katrin Heider, Jing Su, Ymke van der Pol, Mareike Thompson, James Morris, Jonathan C M Wan, Dineika Chandrananda, James Hadfield and 11 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in EMBO molecular medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
75citing papers in PubMed, 3 pooled it
8.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

75 citing papers in PubMed, 3 syntheses or guidelines pooled it, 122 citations in OpenAlex.

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  17. Advances in laboratory medicine · 2025
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15 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors at 2 institutions in 2 countries.

Florent MouliereCancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.ORCID 0000-0001-7043-0514
Christopher G SmithCancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.ORCID 0000-0001-7357-2737
Katrin HeiderCancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.
Jing SuCancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.
Ymke van der PolAmsterdam UMC, Vrije Universiteit Amsterdam, Department of Pathology, Cancer Centre Amsterdam, Amsterdam, The Netherlands.ORCID 0000-0002-3040-5848
Mareike ThompsonCancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.
James MorrisCancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.
Jonathan C M WanCancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.
Dineika ChandranandaCancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.ORCID 0000-0002-8834-9500
James HadfieldCancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.
Marta GrzelakCancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.
Irena HudecovaCancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.
Dominique-Laurent CouturierCancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.
Wendy CooperCancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.ORCID 0000-0003-3416-9982
Hui ZhaoCancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.
Davina GaleCancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.ORCID 0000-0002-4521-8199
Matthew EldridgeCancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.
Colin WattsDivision of Neurosurgery, Department of Clinical Neurosciences, University of Cambridge, Cambridge, UK.
Kevin BrindleCancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.ORCID 0000-0003-3883-6287
Nitzan RosenfeldCancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.ORCID 0000-0002-2825-4788
Richard MairCancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.ORCID 0000-0001-8235-5689
University of Cambridge · GBAmsterdam University Medical Centers · NL

Funding

Cancer Research UK 11906Cancer Research UK 20240Cancer Research UK 27548Cancer Research UK 28289Cancer Research UK 28292Cancer Research UK A16465Cancer Research UK A17197Cancer Research UK A20240Cancer Research UK A29580Department of Health
6 · The paper itself

Abstract

Glioma-derived cell-free DNA (cfDNA) is challenging to detect using liquid biopsy because quantities in body fluids are low. We determined the glioma-derived DNA fraction in cerebrospinal fluid (CSF), plasma, and urine samples from patients using sequencing of personalized capture panels guided by analysis of matched tumor biopsies. By sequencing cfDNA across thousands of mutations, identified individually in each patient's tumor, we detected tumor-derived DNA in the majority of CSF (7/8), plasma (10/12), and urine samples (10/16), with a median tumor fraction of 6.4 × 10

Indexed as

Cell-Free Nucleic AcidsGliomaBiomarkers, TumorHumansLiquid BiopsyMutationPlasmaSequence Analysis, DNABiomarkers, TumorCell-Free Nucleic Acidscell-free DNAcirculating tumor DNAfragmentomicsgliomasliquid biopsy

Identifiers

PMID34291583
PMCPMC8350897
OpenAlexW3183750386

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.