ArticlePlanta2021
Transcriptome analysis identifies putative genes involved in triterpenoid biosynthesis in Platycodon grandiflorus.
Article in Planta, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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Who cites it
21 citing papers in PubMed, 33 citations in OpenAlex.
- Integrated transcriptomic and targeted triterpenoid profiling reveals key enzymes in triterpenoid biosynthesis of Oplopanax elatus.Scientific reports · 2026Article
- Melatonin-inducedFrontiers in plant science · 2026Article
- Full-length transcriptome ofPlant biotechnology (Tokyo, Japan) · 2025Article
- Integrated transcriptome and metabolome provide insight into phenolics and soluble sugar variation in the different varieties ofFrontiers in plant science · 2025Article
- Transcriptome-based analysis of key functional genes in the triterpenoid saponin synthesis pathway of Platycodon grandiflorum.BMC genomic data · 2024Article
- Genome-Wide Identification of the CYP716 Gene Family inPlants (Basel, Switzerland) · 2024Article
- RNA sequencing analysis revealsHorticulture research · 2024Article
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- Enhancing Centelloside Production inPlants (Basel, Switzerland) · 2023Article
- Chromosome-Scale Genome Assembly and Triterpenoid Saponin Biosynthesis in Korean Bellflower (International journal of molecular sciences · 2023Article
- De Novo Transcriptome Sequencing ofInternational journal of molecular sciences · 2023Article
- Transcriptome-wide identification of WRKY transcription factors and their expression profiles in response to methyl jasmonate inPlant signaling & behavior · 2022Article
- Transcriptional Responses for Biosynthesis of Triterpenoids in Exogenous Inducers Treated Inonotus Hispidus Using RNA-Seq.Molecules (Basel, Switzerland) · 2022Article
- Functional characterization of a farnesyl diphosphate synthase from Dendrobium nobile Lindl.AMB Express · 2022Article
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- Conjugate of ibrutinib with a TLR7 agonist suppresses melanoma progression and enhances antitumor immunity.International journal of biological sciences · 2022Article
- Application of High-Throughput Sequencing on the Chinese Herbal Medicine for the Data-Mining of the Bioactive Compounds.Frontiers in plant science · 2022Review
- Molecular Cloning and Functional Characterization of a β-Glucosidase Gene to Produce Platycodin D inFrontiers in plant science · 2022Article
- A Chromosome-Level Reference Genome of Chinese Balloon FlowerFrontiers in genetics · 2022Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
MAIN
conclusionComprehensive transcriptome analysis of different Platycodon grandiflorus tissues discovered genes related to triterpenoid saponin biosynthesis. Platycodon grandiflorus (Jacq.) A. DC. (P. grandiflorus), a traditional Chinese medicine, contains considerable triterpenoid saponins with broad pharmacological activities. Triterpenoid saponins are the major components of P. grandiflorus. Here, single-molecule real-time and next-generation sequencing technologies were combined to comprehensively analyse the transcriptome and identify genes involved in triterpenoid saponin biosynthesis in P. grandiflorus. We quantified four saponins in P. grandiflorus and found that their total content was highest in the roots and lowest in the stems and leaves. A total of 173,354 non-redundant transcripts were generated from the PacBio platform, and three full-length transcripts of β-amyrin synthase, the key synthase of β-amyrin, were identified. A total of 132,610 clean reads obtained from the DNBSEQ platform were utilised to explore key genes related to the triterpenoid saponin biosynthetic pathway in P. grandiflorus, and 96 differentially expressed genes were selected as candidates. The expression levels of these genes were verified by quantitative real-time PCR. Our reliable transcriptome data provide valuable information on the related biosynthesis pathway and may provide insights into the molecular mechanisms of triterpenoid saponin biosynthesis in P. grandiflorus.
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