Evidence map›Paper›PMID 34290273›Full record

ArticleScientific reports2021

Interleukin-27 promotes autophagy in human serum-induced primary macrophages via an mTOR- and LC3-independent pathway.

Sylvain Laverdure, Ziqiu Wang, Jun Yang, Takuya Yamamoto, Tima Thomas, Toyotaka Sato, Kunio Nagashima, Tomozumi Imamichi

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
2.9field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 23 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Sylvain LaverdureLaboratory of Human Retrovirology and Immunoinformatics, Frederick National Laboratory for Cancer Research, Building 550, Room 126, P.O. Box B, Frederick, MD, 21702, USA.
Ziqiu WangElectron Microscope Laboratory, Frederick National Laboratory for Cancer Research, Frederick, MD, 21702, USA.
Jun YangLaboratory of Human Retrovirology and Immunoinformatics, Frederick National Laboratory for Cancer Research, Building 550, Room 126, P.O. Box B, Frederick, MD, 21702, USA.
Takuya YamamotoLaboratory of Immunosenescence, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, 567-0085, Japan.
Tima ThomasLaboratory of Human Retrovirology and Immunoinformatics, Frederick National Laboratory for Cancer Research, Building 550, Room 126, P.O. Box B, Frederick, MD, 21702, USA.
Toyotaka SatoLaboratory of Human Retrovirology and Immunoinformatics, Frederick National Laboratory for Cancer Research, Building 550, Room 126, P.O. Box B, Frederick, MD, 21702, USA.
Kunio NagashimaElectron Microscope Laboratory, Frederick National Laboratory for Cancer Research, Frederick, MD, 21702, USA.
Tomozumi ImamichiLaboratory of Human Retrovirology and Immunoinformatics, Frederick National Laboratory for Cancer Research, Building 550, Room 126, P.O. Box B, Frederick, MD, 21702, USA. timamchi@mail.nih.gov.
Frederick National Laboratory for Cancer Research · USNational Institute of Biomedical Innovation, Health and Nutrition · JP

Funding

CCR NIH HHS HHSN261200800001CNCI NIH HHS HHSN261200800001ENIAID NIH HHS Contract No. HHSN261200800001E
6 · The paper itself

Abstract

Interleukin-27 (IL-27) is a cytokine that suppresses human immunodeficiency virus (HIV)-1 infection in macrophages and is considered as an immunotherapeutic reagent for infectious diseases. It is reported that IL-27 suppresses autophagy in Mycobacterium tuberculosis-infected macrophages; however, a role for IL-27 on autophagy induction has been less studied. In this study, we investigated the impact of IL-27 in both autophagy induction and HIV-1 infection in macrophages. Primary human monocytes were differentiated into macrophages using human AB serum (huAB) alone, macrophage-colony stimulating factor (M-CSF) alone, or a combination of IL-27 with huAB or M-CSF. Electron microscopy and immunofluorescence staining demonstrated that a 20-fold increase in autophagosome formation was only detected in IL-27 + huAB-induced macrophages. Western blot analysis indicated that the autophagosome induction was not linked to either dephosphorylation of the mammalian target of rapamycin (mTOR) or lipidation of microtubule-associated protein 1A/1B-light chain 3 (LC3), an autophagosomal marker, implying that IL-27 can induce autophagy through a novel non-canonical pathway. Here we show for the first time that IL-27 induces autophagy during monocyte-to-macrophage differentiation in a subtype-dependent manner.

Indexed as

Microtubule-Associated ProteinsTOR Serine-Threonine KinasesAutophagyCell DifferentiationCells, CulturedHumansInterleukinsMacrophagesMonocytesSignal TransductionInterleukinsMAP1LC3A protein, humanMicrotubule-Associated ProteinsMTOR protein, humanMYDGF protein, humanTOR Serine-Threonine Kinases

Identifiers

PMID34290273
PMCPMC8295388
OpenAlexW3185717533

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.