ArticleCell reports2021
Heterogeneity of neurons reprogrammed from spinal cord astrocytes by the proneural factors Ascl1 and Neurogenin2.
Article in Cell reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 43 citations in OpenAlex.
- Development of a human iPSC-derived corticospinal tract-on-a-chip.Cell reports methods · 2026Article
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- A proper excitatory/inhibitory ratio is required to develop synchronized network activity in mouse cortical cultures.Stem cell reports · 2025Article
- Astrocyte diversity and subtypes: aligning transcriptomics with multimodal perspectives.EMBO reports · 2025Review
- Development of a Lentiviral Reporter System for In Vitro Reprogramming of Astrocytes to Neuronal Precursors.Biology · 2025Article
- Changing genes, cells and networks to reprogram the brain after stroke.Nature neuroscience · 2025Review
- Hmgb2 improves astrocyte to neuron conversion by increasing the chromatin accessibility of genes associated with neuronal maturation in a proneuronal factor-dependent manner.Genome biology · 2025Article
- Proliferation history and transcription factor levels drive direct conversion to motor neurons.Cell systems · 2025Article
- Lamin A/C regulates cerebellar granule cell maturation.Cell biology and toxicology · 2025Article
- Direct neuronal reprogramming of mouse astrocytes is associated with multiscale epigenome remodeling and requires Yy1.Nature neuroscience · 2024Article
- Article
- Proliferation history and transcription factor levels drive direct conversion.bioRxiv : the preprint server for biology · 2023Article
- The Development of Speaking and Singing in Infants May Play a Role in Genomics and Dementia in Humans.Brain sciences · 2023Review
- Characterizing the targets of transcription regulators by aggregating ChIP-seq and perturbation expression data sets.Genome research · 2023Article
- Knockdown of polypyrimidine tract binding protein facilitates motor function recovery after spinal cord injury.Neural regeneration research · 2023Article
- Engineered neurogenesis in naïve adult rat cortex by Ngn2-mediated neuronal reprogramming of resident oligodendrocyte progenitor cells.Frontiers in neuroscience · 2023Article
- Probing cell identity hierarchies by fate titration and collision during direct reprogramming.Molecular systems biology · 2022Article
- Circuit formation in the adult brain.The European journal of neuroscience · 2022Review
- Prime-seq, efficient and powerful bulk RNA sequencing.Genome biology · 2022Article
Corrections and comments
- Erratum issued
Authors and funding
12 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Astrocytes are a viable source for generating new neurons via direct conversion. However, little is known about the neurogenic cascades triggered in astrocytes from different regions of the CNS. Here, we examine the transcriptome induced by the proneural factors Ascl1 and Neurog2 in spinal cord-derived astrocytes in vitro. Each factor initially elicits different neurogenic programs that later converge to a V2 interneuron-like state. Intriguingly, patch sequencing (patch-seq) shows no overall correlation between functional properties and the transcriptome of the heterogenous induced neurons, except for K-channels. For example, some neurons with fully mature electrophysiological properties still express astrocyte genes, thus calling for careful molecular and functional analysis. Comparing the transcriptomes of spinal cord- and cerebral-cortex-derived astrocytes reveals profound differences, including developmental patterning cues maintained in vitro. These relate to the distinct neuronal identity elicited by Ascl1 and Neurog2 reflecting their developmental functions in subtype specification of the respective CNS region.
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