ArticleJournal of fish diseases2021
Optimization of tools for the detection and identification of Cryptocotyle metacercariae in fish: Digestion method and viability studies.
Article in Journal of fish diseases, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed.
- Prevailing parasitic diseases affectingJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2024Article
- Re-evaluation of certain aspects of the EFSA Scientific Opinion of April 2010 on risk assessment of parasites in fishery products, based on new scientific data. Part 1: ToRs1-3.EFSA journal. European Food Safety Authority · 2024Article
- Black spot diseases in seven commercial fish species from the English Channel and the North Sea: infestation levels, identification and population genetics of Cryptocotyle spp.Parasite (Paris, France) · 2023Article
- Optimization of tools for the detection and identification of Cryptocotyle metacercariae in fish: Digestion method and viability studies.Journal of fish diseases · 2021Article
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5 authors.
Funding
Abstract
Some trematode metacercariae, including marine digeneans belonging to the genus Cryptocotyle, induce black spots in target tissues due to the attraction of fish host melanophores. To promote precise quantification of infection, the counting of black spots has to be confirmed by reliable quantification of metacercariae after tissue digestion. This process ensures the isolation of undamaged parasites for morphological and molecular identification. The aim of this work was to optimize the pepsin digestion protocol and to assess the duration of viability of Cryptocotyle metacercariae in fish post-mortem (pm). Four digestion protocols were compared by measuring the viability rate of metacercariae. The present study shows that the orbital digestion method was the least destructive for metacercariae and allowed better quantification of Cryptocotyle infection. Moreover, morphological identification seemed reliable up to 8 days pm for Cryptocotyle infection.
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