Evidence map›Paper›PMID 34289076›Full record

ArticleBiotechnology and bioengineering2021

Engineered acetoacetate-inducible whole-cell biosensors based on the AtoSC two-component system.

Jack W Rutter, Linda Dekker, Alex J H Fedorec, David T Gonzales, Ke Yan Wen, Lewis E S Tanner, Emma Donovan, Tanel Ozdemir, Geraint M Thomas, Chris P Barnes

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology and bioengineering, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Article
  3. ACS omega · 2023
    Review
  4. Article
  5. Article
  6. Article
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Jack W RutterDepartment of Cell and Developmental Biology, University College London, London, UK.
Linda DekkerDepartment of Cell and Developmental Biology, University College London, London, UK.
Alex J H FedorecDepartment of Cell and Developmental Biology, University College London, London, UK.
David T GonzalesDepartment of Cell and Developmental Biology, University College London, London, UK.
Ke Yan WenDepartment of Cell and Developmental Biology, University College London, London, UK.
Lewis E S TannerDepartment of Cell and Developmental Biology, University College London, London, UK.
Emma DonovanDepartment of Cell and Developmental Biology, University College London, London, UK.
Tanel OzdemirDepartment of Cell and Developmental Biology, University College London, London, UK.
Geraint M ThomasDepartment of Cell and Developmental Biology, University College London, London, UK.
Chris P BarnesDepartment of Cell and Developmental Biology, University College London, London, UK.

Funding

Biotechnology and Biological Sciences Research Council BB/S003681/1European Research Council 770835Wellcome Trust 209409Wellcome Trust 209409/Z/17/Z
6 · The paper itself

Abstract

Whole-cell biosensors hold potential in a variety of industrial, medical, and environmental applications. These biosensors can be constructed through the repurposing of bacterial sensing mechanisms, including the common two-component system (TCS). Here we report on the construction of a range of novel biosensors that are sensitive to acetoacetate, a molecule that plays a number of roles in human health and biology. These biosensors are based on the AtoSC TCS. An ordinary differential equation model to describe the action of the AtoSC TCS was developed and sensitivity analysis of this model used to help inform biosensor design. The final collection of biosensors constructed displayed a range of switching behaviours at physiologically relevant acetoacetate concentrations and can operate in several Escherichia coli host strains. It is envisaged that these biosensor strains will offer an alternative to currently available commercial strip tests and, in future, may be adopted for more complex in vivo or industrial monitoring applications.

Indexed as

Biosensing TechniquesEscherichia coliEscherichia coli ProteinsGene Expression Regulation, BacterialAcetoacetatesHumansOperonAcetoacetatesacetoacetic acidEscherichia coli ProteinsacetoacetatebiosensorsE. colisynthetic biologytwo-component systems

Identifiers

PMID34289076
PMCPMC7618054

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.