Evidence map›Paper›PMID 34288729›Full record

ArticleJournal of clinical microbiology2021

A Simple Reverse Transcriptase PCR Melting-Temperature Assay To Rapidly Screen for Widely Circulating SARS-CoV-2 Variants.

Padmapriya Banada, Raquel Green, Sukalyani Banik, Abby Chopoorian, Deanna Streck, Robert Jones, Soumitesh Chakravorty, David Alland

Abstract read
In one paragraph

Article in Journal of clinical microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.

0numbers the graph read from it
0cells of the map it votes in
42citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

42 citing papers in PubMed.

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  10. [Rapid detection and genotyping of SARS-CoV-2 Omicron BA.4/5 variants using a RT-PCR and CRISPR-Cas12a-based assay].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2023
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Padmapriya BanadaPublic Health Research Institute, Center for Emerging Pathogens, Rutgers New Jersey Medical School, Newark, New Jersey, USA.ORCID 0000-0002-5217-6142
Raquel GreenPublic Health Research Institute, Center for Emerging Pathogens, Rutgers New Jersey Medical School, Newark, New Jersey, USA.
Sukalyani BanikPublic Health Research Institute, Center for Emerging Pathogens, Rutgers New Jersey Medical School, Newark, New Jersey, USA.
Abby ChopoorianPublic Health Research Institute, Center for Emerging Pathogens, Rutgers New Jersey Medical School, Newark, New Jersey, USA.
Deanna StreckInstitute of Genomic Medicine, Rutgers New Jersey Medical School, Newark, New Jersey, USA.
Robert JonesCraic Computing LLC, Snohomish, Washington, USA.
Soumitesh ChakravortyPublic Health Research Institute, Center for Emerging Pathogens, Rutgers New Jersey Medical School, Newark, New Jersey, USA.
David AllandPublic Health Research Institute, Center for Emerging Pathogens, Rutgers New Jersey Medical School, Newark, New Jersey, USA.ORCID 0000-0001-5196-1571

Funding

Resources, Workforce Development, and Animal Models for the Rutgers RBLUC7AI180307 · NIAID · RUTGERS BIOMEDICAL AND HEALTH SCIENCES · PI Padmini Salgame · 2023 to 2026
$20.1M
TB detection in children using a novel stool-based GeneXpert assayR01AI131617 · NIAID · RBHS-NEW JERSEY MEDICAL SCHOOL · PI ALLAND, DAVID, NICOL, MARK PATRICK · 2018 to 2022
$2.9M
NIAID NIH HHS R01 AI131617NIAID NIH HHS UC7 AI180307
6 · The paper itself

Abstract

The increased transmission of SARS-CoV-2 variants of concern (VOC), which originated in the United Kingdom (B.1.1.7/alpha), South Africa (B1.351/beta), Brazil (P.1/gamma), the United States (B.1.427/429 or epsilon), and India (B.1.617.2/delta), requires a vigorous public health response, including real-time strain surveillance on a global scale. Although genome sequencing is the gold standard for identifying these VOCs, it is time-consuming and expensive. Here, we describe a simple, rapid, and high-throughput reverse transcriptase PCR (RT-PCR) melting-temperature (

Indexed as

COVID-19SARS-CoV-2HumansReverse Transcriptase Polymerase Chain ReactionTemperatureE484Kmelting temperatureN501YSARS-CoV-2screening testsloppy molecular beaconvariants

Identifiers

PMID34288729
PMCPMC8451443

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.