Evidence map›Paper›PMID 34288603›Full record

ArticleBiotechnology progress2021

Probing morphological, genetic and metabolomic changes of in vitro embryo development in a microfluidic device.

Vanessa Mancini, Paul J McKeegan, Alexandra C Schrimpe-Rutledge, Simona G Codreanu, Stacy D Sherrod, John A McLean, Helen M Picton, Virginia Pensabene

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology progress, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Vanessa ManciniSchool of Electronic and Electrical Engineering, University of Leeds, Leeds, UK.
Paul J McKeeganReproduction and Early Development Research Group, Discovery and Translational Science Department, Leeds Institute of Cardiovascular and Metabolic Medicine, School of Medicine, University of Leeds, UK.
Alexandra C Schrimpe-RutledgeCenter for Innovative Technology (CIT), Department of Chemistry, Vanderbilt University, Nashville, Tennessee, USA.
Simona G CodreanuCenter for Innovative Technology (CIT), Department of Chemistry, Vanderbilt University, Nashville, Tennessee, USA.
Stacy D SherrodCenter for Innovative Technology (CIT), Department of Chemistry, Vanderbilt University, Nashville, Tennessee, USA.
John A McLeanCenter for Innovative Technology (CIT), Department of Chemistry, Vanderbilt University, Nashville, Tennessee, USA.
Helen M PictonReproduction and Early Development Research Group, Discovery and Translational Science Department, Leeds Institute of Cardiovascular and Metabolic Medicine, School of Medicine, University of Leeds, UK.
Virginia PensabeneSchool of Electronic and Electrical Engineering, University of Leeds, Leeds, UK.ORCID 0000-0002-3352-8202

Funding

Medical Research Council MC_PC_17165National Centre for the Replacement, Refinement and Reduction of Animals in Research NC/C016S02/1
6 · The paper itself

Abstract

Assisted reproduction technologies for clinical and research purposes rely on a brief in vitro embryo culture which, despite decades of progress, remain suboptimal in comparison to the physiological environment. One promising tool to improve this technique is the development of bespoke microfluidic chambers. Here we present and validate a new microfluidic device in polydimethylsiloxane (PDMS) for the culture of early mouse embryos. Device material and design resulted embryo compatible and elicit minimal stress. Blastocyst formation, hatching, attachment and outgrowth formation on fibronectin-coated devices were similar to traditional microdrop methods. Total blastocyst cell number and allocation to the trophectoderm and inner cell mass lineages were unaffected. The devices were designed for culture of 10-12 embryos. Development rates, mitochondrial polarization and metabolic turnover of key energy substrates glucose, pyruvate and lactate were consistent with groups of 10 embryos in microdrop controls. Increasing group size to 40 embryos per device was associated with increased variation in development rates and altered metabolism. Device culture did not perturb blastocyst gene expression but did elicit changes in embryo metabolome, which can be ascribed to substrate leaching from PDMS and warrant further investigation.

Indexed as

BlastocystLab-On-A-Chip DevicesReproductive Techniques, AssistedAnimalsEmbryonic DevelopmentMetabolomeMetabolomicsMiceassisted reproductive technologiesembryo cultureIVFmicemicrofluidics

Identifiers

PMID34288603
PMCPMC11475506

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.