Evidence map›Paper›PMID 34287037›Full record

ArticleJournal of virology2021

Structural Determinants of Virion Assembly and Release in the C Terminus of the Mason-Pfizer Monkey Virus Capsid Protein.

Marlene V Buckmaster, Kaneil K Zadrozny, Barbie K Ganser-Pornillos, Owen Pornillos, Stephen P Goff

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 56% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Exosomal transmission of viruses, a two-edged biological sword.Cell communication and signaling : CCS · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Marlene V BuckmasterDepartment of Biological Sciences, Columbia University, New York, New York, USA.
Kaneil K ZadroznyDepartment of Molecular Physiology and Biological Physics, University of Virginiagrid.27755.32, Charlottesville, Virginia, USA.
Barbie K Ganser-PornillosDepartment of Molecular Physiology and Biological Physics, University of Virginiagrid.27755.32, Charlottesville, Virginia, USA.
Owen PornillosDepartment of Molecular Physiology and Biological Physics, University of Virginiagrid.27755.32, Charlottesville, Virginia, USA.
Stephen P GoffHHMI, Columbia University Medical Center, New York, New York, USA.ORCID 0000-0002-9679-0582
University of Virginia · USColumbia University Irving Medical Center · US

Funding

Mechanisms of Silencing of Retroviral DNAs in Embryonic Cell LinesR01CA030488 · NCI · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI GOFF, STEPHEN PAINE · 1985 to 2024
$4.3M
Molecular mechanisms of the CA-SP1 switch in HIV assembly and maturationR01AI129678 · NIAID · UNIVERSITY OF VIRGINIA · PI GANSER-PORNILLOS, BARBIE K., PORNILLOS, OWEN · 2017 to 2021
$2.3M
HHS | National Institutes of Health (NIH) R01 CA 030488Howard Hughes Medical Institute (HHMI) NANational Science Foundation (NSF) GRFP grant #1644869NCI NIH HHS R01 CA030488NIAID NIH HHS R01 AI129678
6 · The paper itself

Abstract

The transition from an immature to a fully infectious mature retrovirus particle is associated with molecular switches that trigger dramatic conformational changes in the structure of the Gag proteins. A dominant maturation switch that stabilizes the immature capsid (CA) lattice is located downstream of the CA protein in many retroviral Gags. The HIV-1 Gag protein contains a stretch of 5 amino acid residues termed the "clasp motif," important for the organization of the hexameric subunits that provide stability to the overall immature HIV-1 shell. Sequence alignment of the CA C-terminal domains (CTDs) of HIV-1 and Mason-Pfizer monkey virus (M-PMV) highlighted a spacer-like domain in M-PMV that may provide a comparable function. The importance of the sequences spanning the CA-nucleocapsid (NC) cleavage has been demonstrated by mutagenesis, but the specific requirements for the clasp motif in several steps of M-PMV particle assembly and maturation have not been determined in detail. In the present study, we report an examination of the role of the clasp motif in the M-PMV life cycle. We generated a series of M-PMV Gag mutants and assayed for assembly of the recombinant proteins

Indexed as

Virus AssemblyAmino Acid MotifsCapsid ProteinsCryoelectron MicroscopyGene Products, gagGenome, ViralHEK293 CellsHeLa CellsHumansMason-Pfizer monkey virusMutant ProteinsProtein DomainsRNA, ViralViral Genome PackagingVirionCapsid ProteinsGene Products, gagMutant ProteinsRNA, ViralclaspM-PMV capsidM-PMV infectivityM-PMV particle releaseretrovirus assembly

Identifiers

PMID34287037
PMCPMC8428407
OpenAlexW3187072755

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.