ArticlemSphere2021
Amplicon Sequence Variants Artificially Split Bacterial Genomes into Separate Clusters.
Article in mSphere, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 97 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
97 citing papers in PubMed, 1 synthesis or guideline pooled it, 180 citations in OpenAlex.
- The emerging role of the urinary microbiome in benign noninfectious urological conditions: an up-to-date systematic review.World journal of urology · 2023Pooled it
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- Soil Resistomes in a Tropical Watershed are Indirectly Structured by Bacterial Community Interactions with Soil Properties.bioRxiv : the preprint server for biology · 2026Article
- Alterations in gut microbiota composition in children with methylmalonic acidemia, propionic acidemia, and maple syrup urine disease.European journal of clinical nutrition · 2026Article
- Timing Is Everything: The Effect of Exposure to Pollution on Wildlife Gut Microbiota Is Contingent on Season.Molecular ecology · 2026Article
- Rare taxa enhance microbial network complexity and drive nitrification and denitrification processes in river ecosystems.Environmental microbiome · 2026Article
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- Microbial Communities Vary by Body Region but Remain Consistent Across Color Morphs in the Solitary AscidianInternational journal of microbiology · 2026Article
- Microbiome stewardship: definition and guiding principles for implementation.Sustainable microbiology · 2026Article
- Synthetic rhizosphere bacterial communities induce systemic resistance to barley powdery mildew without major shifts in the native bacterial community.Frontiers in microbiology · 2026Article
- CoSMIC: A hybrid approach for large-scale, high-resolution microbial profiling of novel niches.PloS one · 2026Article
- From farm to microbe: organic amendments and soil texture as drivers of soil Microbiome composition.Environmental microbiome · 2025Article
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- Age and anatomical site shape the gastrointestinal bacterial communities of captive American crocodiles (Crocodylus acutus).Folia microbiologica · 2025Article
- Associations of Salivary Microbiota with Diet Quality, Body Mass Index, and Oral Health Status in Turkish Adolescents.Nutrients · 2025Article
- Quantification of major bacterial pathogens in liver abscesses and matched ruminal and colonic epithelial tissues of feedlot cattle.Microbiology spectrum · 2025Article
- Bacterial 16S Ribosomal Gene Fingerprints as a Tool to Diagnose and Mitigate Fish Larvae Gut Dysbiosis.Environmental microbiology reports · 2025Article
37 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
Amplicon sequencing variants (ASVs) have been proposed as an alternative to operational taxonomic units (OTUs) for analyzing microbial communities. ASVs have grown in popularity, in part because of a desire to reflect a more refined level of taxonomy since they do not cluster sequences based on a distance-based threshold. However, ASVs and the use of overly narrow thresholds to identify OTUs increase the risk of splitting a single genome into separate clusters. To assess this risk, I analyzed the intragenomic variation of 16S rRNA genes from the bacterial genomes represented in an
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.