Evidence map›Paper›PMID 34286295›Full record

ArticleSTAR protocols2021

Bioluminescent optogenetic (BL-OG) activation of neurons during mouse postnatal brain development.

Emmanuel L Crespo, Mansi Prakash, Andreas Bjorefeldt, William E Medendorp, Nathan C Shaner, Diane Lipscombe, Christopher I Moore, Ute Hochgeschwender

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.5field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. CaBLAM! A high-contrast bioluminescent CabioRxiv : the preprint server for biology · 2025
    Article
  3. Article
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Emmanuel L CrespoBiochemistry, Cellular and Molecular Biology Graduate program, Central Michigan University, Mount Pleasant, MI 48859, USA.
Mansi PrakashCollege of Medicine, Central Michigan University, Mount Pleasant, MI 48859, USA.
Andreas BjorefeldtCollege of Medicine, Central Michigan University, Mount Pleasant, MI 48859, USA.
William E MedendorpNeuroscience Graduate Program, Central Michigan University, Mount Pleasant, MI 48859, USA.
Nathan C ShanerDepartment of Neuroscience, University of California San Diego, La Jolla, CA, USA.
Diane LipscombeCarney Institute for Brain Science, Brown University, Providence, RI 02906, USA.
Christopher I MooreCarney Institute for Brain Science, Brown University, Providence, RI 02906, USA.
Ute HochgeschwenderBiochemistry, Cellular and Molecular Biology Graduate program, Central Michigan University, Mount Pleasant, MI 48859, USA.
Central Michigan University · USAllen Institute for Brain Science · USUniversity of California San Diego · US

Funding

BioLuminescent OptoGenetics (BL-OG): A Novel and Versatile Strategy for NeuromodulationU01NS099709 · NINDS · CENTRAL MICHIGAN UNIVERSITY · PI HOCHGESCHWENDER, UTE H, MOORE, CHRISTOPHER I · 2016 to 2018
$2.4M
New tools for quantitative non-invasive recording of biochemical signalsR01CA279813 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Jennifer Prescher, Nathan Christopher Shaner · 2024 to 2026
$2.0M
Genetically Encoded Light-Production and Light-Sensing for Neuronal ManipulationR21MH101525 · NIMH · DUKE UNIVERSITY · PI HOCHGESCHWENDER, UTE H · 2013 to 2015
$434k
NCI NIH HHS R01 CA279813NIMH NIH HHS R21 MH101525NINDS NIH HHS U01 NS099709
6 · The paper itself

Abstract

Bioluminescent optogenetics (BL-OG) allows activation of photosensory proteins, such as opsins, by either fiberoptics or by administering a luciferin. BL-OG thus confers both optogenetic and chemogenetic access within the same genetically targeted neuron. This bimodality offers a powerful approach for non-invasive chemogenetic manipulation of neural activity during brain development and adult behaviors with standard optogenetic spatiotemporal precision. We detail protocols for bioluminescent stimulation of neurons in postnatally developing brain and its validation through bioluminescence imaging and electrophysiological recording in mice. For complete information on the use and execution of this protocol, please refer to Medendorp et al. (2021).

Indexed as

BrainNeuronsAnimalsElectrophysiological PhenomenaElectrophysiologyLuminescent MeasurementsMiceOptical ImagingOptogeneticsPatch-Clamp TechniquesBiotechnology and bioengineeringDevelopmental biologyMicroscopyModel OrganismsMolecular BiologyNeuroscience

Identifiers

PMID34286295
PMCPMC8273415
OpenAlexW3179763655

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.