ArticleScientific reports2021
Validation of real-time RT-PCR for detection of SARS-CoV-2 in the early stages of the COVID-19 outbreak in the Republic of Korea.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 52 citations in OpenAlex.
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- Diagnostic value comparative analysis of the commercial kits for the detection of SARS-CoV-2 in clinical samples: a systematic review and meta-analysis.Iranian journal of microbiology · 2025Review
- High Concordance Between SYBR Green and TaqMan PCR for SARS-CoV-2 Detection in Nasopharyngeal and Saliva Samples.Viruses · 2025Article
- Using Machine Learning and Optical Microscopy Image Analysis of Immunosensors Made on Plasmonic Substrates: Application to Detect the SARS-CoV-2 Virus.ACS sensors · 2025Article
- An integrated methodological framework for the validation and verification of clinical testing by qRT-PCR.Heliyon · 2025Article
- Development of a new quantitative RT-PCR to detect lymphocytic choriomeningitis virus.Frontiers in veterinary science · 2025Article
- Antibacterial Activity of Silver-Modified CuO Nanoparticle-Coated Masks.Bioengineering (Basel, Switzerland) · 2024Article
- Serological survey in a university community after the fourth wave of COVID-19 in Senegal.PloS one · 2024Article
- Stability of ACE2 Peptide Mimetics and Their Implications on the Application for SARS-CoV2 Detection.Biosensors · 2023Article
- Re: Vitamin D deficiency predicts 30-day hospital mortality of adults with COVID-19.Clinical nutrition ESPEN · 2023Article
- Phenylpyrazolone-1,2,3-triazole Hybrids as Potent Antiviral Agents with Promising SARS-CoV-2 Main Protease Inhibition Potential.Pharmaceuticals (Basel, Switzerland) · 2023Article
- Detection of COVID-19-related biomarkers by electrochemical biosensors and potential for diagnosis, prognosis, and prediction of the course of the disease in the context of personalized medicine.Analytical and bioanalytical chemistry · 2023Review
- Peptide-based direct electrochemical detection of receptor binding domains of SARS-CoV-2 spike protein in pristine samples.Sensors and actuators. B, Chemical · 2023Article
- Rapid antigen test as a tool for the identification of SARS-CoV-2 infection and its potential as a self-testing device.Revista da Sociedade Brasileira de Medicina Tropical · 2023Article
- A novel study on bean common mosaic virus accumulation shows disease resistance at the initial stage of infection inFrontiers in genetics · 2023Article
- Detection of Recombinant Proteins SOX2 and OCT4 Interacting in HEK293T Cells Using Real-Time Quantitative PCR.Life (Basel, Switzerland) · 2022Article
- Highly Adsorptive Au-TiOACS applied materials & interfaces · 2022Article
- Article
- Rapid identification of SARS-CoV-2 in the point-of-care using digital PCR-based Dr. PCR™ Di20K COVID-19 Detection Kit without viral RNA extraction.Genes & genomics · 2022Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
A real-time reverse transcription polymerase chain reaction (RT-qPCR) assay that does not require Emergency Use Authorization (EUA) reagents was tested and validated for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) during the early stages of the outbreak of coronavirus disease 2019 (COVID-19) in the Republic of Korea. Early diagnosis of COVID-19 enables timely treatment and the implementation of public health measures. We validated the sensitivity, specificity, precision, linearity, accuracy, and robustness of the RT-qPCR assay for SARS-CoV-2 detection and compared its performance with that of several EUA-approved kits. Our RT-qPCR assay was highly specific for SARS-CoV-2 as demonstrated by not amplifying 13 other viruses that cause respiratory diseases. The assay showed high linearity using a viral isolate from a patient with known COVID-19 as well as plasmids containing target SARS-CoV-2 genes as templates. The assay showed good repeatability and reproducibility with a coefficient of variation of 3%, and a SARS-CoV-2 limit of detection of 1 PFU/mL. The RT-qPCR-based assay is highly effective and can facilitate the early diagnosis of COVID-19 without the use of EUA-approved kits or reagents in the Republic of Korea.
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