ArticleEuropean journal of histochemistry : EJH2021
Efficacy of immunohistochemical staining in detecting <em>Helicobacter pylori</em> in Saudi patients with minimal and atypical infection.
Article in European journal of histochemistry : EJH, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 15 citations in OpenAlex.
- Assessing the Malignant Potential of Gastric Mucosa Disorders: A Pilot Study on Lipid Peroxidation End Products and COX-2 Detection by Immunohistochemistry.Molecular carcinogenesis · 2026Article
- Species-level dynamics of gastric microbiome after Helicobacter pylori eradication in high-risk Mongolian population.Gut pathogens · 2026Article
- Optimal post-gastroscopy timing of the urea [Frontiers in medicine · 2026Article
- Rapid and Efficient Screening ofDiagnostics (Basel, Switzerland) · 2025Article
- Optical Biosensor Based on Porous Silicon and Tamm Plasmon Polariton for Detection of CagA Antigen ofSensors (Basel, Switzerland) · 2024Article
- Pathohistological Changes in the Gastric Mucosa in Correlation with the Immunohistochemically Detected Spiral and Coccoid Forms ofMicroorganisms · 2024Article
- Epidemiology, pathogenicity, risk factors, and management ofBiomolecules & biomedicine · 2024Review
- Review
- 25-hydroxyvitamin D levels in children of different ages and with varying degrees ofFrontiers in pediatrics · 2023Article
- Usefulness of Molecular Methods forInternational journal of molecular sciences · 2022Article
- The high sensitivity and specificity of rapid urease test in diagnosis ofIranian journal of microbiology · 2022Article
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gastric Helicobacter pylori infection is diagnosed based on histopathological evaluation of gastric mucosal biopsies, urease test, urea breath test, H. pylori culturing, or direct detection using polymerase chain reaction (PCR). This study aimed to evaluate the efficacy of immunohistochemical (IHC) staining in detecting H. pylori in gastric biopsies from patients with chronic gastritis and minimal or atypical infection. Gastric biopsies from 50 patients with chronic gastritis were subjected to routine haematoxylin and eosin (H-E), modified Giemsa, and IHC staining. The results of staining were compared with those of quantitative real-time PCR (qRT-PCR). The qRT-PCR analysis identified 32 (64%) H. pylori-positive cases, whereas IHC, H-E, and modified Giemsa staining identified 29 (58%), 27 (54%), and 21 (42%) positive cases. The sensitivity of IHC staining (87.50%) was higher than that of H-E (59.38%) and modified Giemsa (43.75%) staining. The specificity of H-E, modified Giemsa, and IHC staining was 55.56%, 61.11%, and 94.44%, respectively. IHC staining exhibited the highest diagnostic accuracy (90%), followed by H-E (58%) and modified Giemsa (50%) staining. Active gastritis, intestinal metaplasia, and lymphoid follicles were detected in 32 (64%), 4 (8%), and 22 (44%) cases, respectively, and all of these cases were H. pylori positive. In contrast to routine H-E and modified Giemsa staining, IHC allows for the accurate H. pylori detection in cases with minimal or atypical infection. Moreover, IHC can be an alternative diagnostic method to qRT-PCR for detection of H. pylori in such cases.
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