ArticleJournal of experimental botany2021
Derepression of specific miRNA-target genes in rice using CRISPR/Cas9.
Article in Journal of experimental botany, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- Sulfur Homeostasis in Rice as a Dynamic Regulatory Network: Functional Genomics, Metabolic Crosstalk, and miR395-Mediated Control.Molecular biotechnology · 2026Review
- Harnessing microRNA regulatory networks for engineering climate resilience and nutritional enhancement in millets.Stress biology · 2026Review
- The multifaceted regulatory network of microRNA396: Orchestrating plant plasticity through coordinating development and various stresses.Journal of integrative plant biology · 2026Review
- Small but big player: the important role of microRNAs in rice.Frontiers in plant science · 2026Review
- Regulatory Landscapes of Non-Coding RNAs During Drought Stress in Plants.International journal of molecular sciences · 2025Review
- Conditional knockdown of gene expression in plants via 3' UTR editing.Plant communications · 2025Article
- MicroRNAs as potent regulators in nitrogen and phosphorus signaling transduction and their applications.Stress biology · 2024Review
- Review
- miRNA mediated regulation of nitrogen response and nitrogen use efficiency of plants: the case of wheat.Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Using CRISPR/Cas to enhance gene expression for crop trait improvement by editing miRNA targets.Journal of experimental botany · 2023Article
- Regulatory Small RNAs for a Sustained Eco-Agriculture.International journal of molecular sciences · 2023Review
- Genome-wide identification ofPeerJ · 2023Article
- Multiple Functions of MiRNAs inLife (Basel, Switzerland) · 2022Review
- CRISPR/Cas9-Mediated Knockout of miR-130b Affects Mono- and Polyunsaturated Fatty Acid Content via PPARG-PGC1α Axis in Goat Mammary Epithelial Cells.International journal of molecular sciences · 2022Article
- Biogenesis, Trafficking, and Function of Small RNAs in Plants.Frontiers in plant science · 2022Article
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs) target specific mRNA molecules based on sequence complementarity for their degradation or repression of translation, thereby regulating various developmental and physiological processes in eukaryotic organisms. Expressing the target mimicry (MIM) and short tandem target mimicry (STTM) can block endogenous activity of mature miRNAs and eliminate the inhibition of their target genes, resulting in phenotypic changes due to higher expression of the target genes. Here, we report a strategy to achieve derepression of interested miRNA-target genes through CRISPR/Cas9-based generation of in-frame mutants within the miRNA-complementary sequence of the target gene. We show that two rice genes, OsGRF4 (GROWTH REGULATING FACTOR 4) and OsGRF8 carrying in-frame mutants with disruption of the miR396 recognition sites, escape from miR396-mediated post-transcriptional silencing, resulting in enlarged grain size and increase in brown planthopper (BPH) resistance, in their respective transgenic rice lines. These results demonstrate that CRISPR/Cas9-mediated disruption of miRNA target sites can be effectively employed to precisely derepress particular target genes of functional importance for trait improvement in plants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.