ArticlemSystems2021
Comprehensive Cultivation of the Swine Gut Microbiome Reveals High Bacterial Diversity and Guides Bacterial Isolation in Pigs.
Article in mSystems, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 35 citations in OpenAlex.
- Effect of bovine respiratory disease on the respiratory microbiome: a meta-analysis.Frontiers in cellular and infection microbiology · 2023Pooled it
- Bridging the gap:Gut microbes · 2026Review
- Genome-resolved and culture-based atlas of the feline gut microbiome enables host-adapted probiotic development.NPJ biofilms and microbiomes · 2026Article
- Feeding of Undocked Pigs: Effects of Feed Components and Feed Composition.Animals : an open access journal from MDPI · 2026Review
- Exploring the predominant factors associated with shaping the cultivable swine gut microbiota of Wuzhishan piglets under in vitro cultivation conditions.BMC microbiology · 2026Article
- Copper is an intestinal habitat filter affecting the gut microbiota interactions with Salmonella Typhimurium.Microbiome · 2026Article
- Lactic acid bacteria isolated from mammalian feces exhibit distinct diversity and probiotic traits.World journal of microbiology & biotechnology · 2026Article
- A culturomics approach reveals cross-feeding capacity of intestinal pig bacteria upon release of inositol from phytate.Microbiome · 2026Article
- Metagenomic analysis of fecal and environmental microbiota in rural mixed livestock farming systems in South Africa.Frontiers in cellular and infection microbiology · 2026Article
- Intestinal microbiota dynamics in piglets: the interplay with swine enteric coronavirus infections and implications for disease control.Animal microbiome · 2025Review
- Structure-dependent degradation of milk oligosaccharides by newly isolated intestinal commensal bacterial strains from suckling piglets and rabbits.BMC microbiology · 2025Article
- Genomic characterization of antimicrobial resistance and mobile genetic elements in swine gut bacteria isolated from a Canadian research farm.Animal microbiome · 2025Article
- Integrating the milk microbiome signatures in mastitis: milk-omics and functional implications.World journal of microbiology & biotechnology · 2025Review
- Gallibacterium faecale sp. nov., Isolated from Dairy Cow Feces.Current microbiology · 2025Article
- Analysis of causal pathogens of mulberry bacterial blight in samples collected from eight provinces of China using culturomics and metagenomic sequencing methods.Frontiers in plant science · 2025Article
- Fecal microbiome of pigs fed diets differing in protein and amino acid content raised in thermoneutral or cyclical heat stress conditions.Frontiers in microbiology · 2025Article
- Multi-omics analysis reveals the core microbiome and biomarker for nutrition degradation in alfalfa silage fermentation.mSystems · 2024Article
- The giant panda gut harbors a high diversity of lactic acid bacteria revealed by a novel culturomics pipeline.mSystems · 2024Article
- Dispersion of antimicrobial resistant bacteria in pig farms and in the surrounding environment.Animal microbiome · 2024Article
- Characterization of the Effects of a Novel Probiotic onAnimals : an open access journal from MDPI · 2024Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
Abstract
Despite the substantial progress made in human gut culturomics, little is known about the culturability of the swine gut microbiota. In this study, we cultured swine gut microbiota using 53 bacterial cultivation methods with different medium and gas combinations from three pigs at four different growth stages. Both culture-dependent (CD; colony mixtures from each method) and culture-independent (CI; original fecal suspensions) samples were subjected to 16S rRNA gene amplicon sequencing. Increasing microbial diversities were observed in both CI and CD samples from successive growth stages. While a total of 378, 482, 565, and 555 bacterial amplicon sequence variants (ASVs) were observed in the CI samples, higher microbial diversities (415, 675, 808, and 823 observed ASVs) were detected using the CD methods at the lactation, nursery, growing, and finishing stages, respectively. We constructed reference culture maps showing the preferred cultivation conditions for specific bacterial taxa and examined the effects of culturing factors such as oxygen, medium, donor pig age, antibiotics, and blood culture preincubation on swine gut microbiota cultivation. We focused on a wide range of beneficial bacteria, chose 1,299 colonies based on the reference map, and Sanger sequenced their 16S rRNA genes. These isolates clustered into 148 different bacterial taxa covering 28 genera. We observed 11, 19, 33, and 25 pairs of cooccurring ASVs in both CD and CI samples at four successive growth stages. This study provides guidance in culturing the swine gut microbiota of interest, which is critical when characterizing their functions in this important animal species.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.