ArticleeLife2021
Linking plasmid-based beta-lactamases to their bacterial hosts using single-cell fusion PCR.
Article in eLife, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
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- Dissecting microbial communities with single-cell transcriptome analysis.Science (New York, N.Y.) · 2025Review
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- Emerging tools for uncovering genetic and transcriptomic heterogeneities in bacteria.Biophysical reviews · 2024Review
- Massively parallel single-cell sequencing of diverse microbial populations.Nature methods · 2024Article
- Functional, transcriptomic, and lipidomic studies of theMicrobiology spectrum · 2024Article
- Plasmids, a molecular cornerstone of antimicrobial resistance in the One Health era.Nature reviews. Microbiology · 2024Review
- Clinically relevant antibiotic resistance genes are linked to a limited set of taxa within gut microbiome worldwide.Nature communications · 2023Article
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- Wastewater surveillance of antibiotic-resistant bacterial pathogens: A systematic review.Frontiers in microbiology · 2022Review
- Linking plasmid-based beta-lactamases to their bacterial hosts using single-cell fusion PCR.eLife · 2021Article
- Examining horizontal gene transfer in microbial communities.Nature reviews. Microbiology · 2021Review
- Managing the Transition to Widespread Metagenomic Monitoring: Policy Considerations for Future Biosurveillance.Health securityArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The horizonal transfer of plasmid-encoded genes allows bacteria to adapt to constantly shifting environmental pressures, bestowing functional advantages to their bacterial hosts such as antibiotic resistance, metal resistance, virulence factors, and polysaccharide utilization. However, common molecular methods such as short- and long-read sequencing of microbiomes cannot associate extrachromosomal plasmids with the genome of the host bacterium. Alternative methods to link plasmids to host bacteria are either laborious, expensive, or prone to contamination. Here we present the One-step Isolation and Lysis PCR (OIL-PCR) method, which molecularly links plasmid-encoded genes with the bacterial 16S rRNA gene via fusion PCR performed within an emulsion. After validating this method, we apply it to identify the bacterial hosts of three clinically relevant beta-lactamases within the gut microbiomes of neutropenic patients, as they are particularly vulnerable multidrug-resistant infections. We successfully detect the known association of a multi-drug resistant plasmid with
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.