ArticleProceedings of the National Academy of Sciences of the United States of America2021
Tradeoffs for a viral mutant with enhanced replication speed.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- Open questions in enterovirus uncoating.Journal of virology · 2026Review
- A single amino acid mutation in norovirus NS4 promotes viral spread.Journal of virology · 2026Article
- Genomic characterization and evolutionary analysis of Chikungunya virus strains in Guangzhou, China, 2025.Virology journal · 2025Article
- Egress-enhancing mutation reveals the inefficiency of non-enveloped virus cell exit.PLoS biology · 2025Article
- Article
- Adaptive truncation of the S gene in IBV during chicken embryo passaging plays a crucial role in its attenuation.PLoS pathogens · 2024Article
- Murine norovirus mutants adapted to replicate in human cells reveal a post-entry restriction.Journal of virology · 2024Article
- Murine norovirus mutants adapted to replicate in human cells reveal a post-entry restriction.bioRxiv : the preprint server for biology · 2024Article
- Human placental cells are resistant to SARS-CoV-2 infection and replication.Wellcome open research · 2024Article
- Enhanced detection and molecular modeling of adaptive mutations in SARS-CoV-2 coding and non-coding regions using the c/µ test.Virus evolution · 2024Article
- Attenuation hotspots in neurotropic human astroviruses.PLoS biology · 2023Article
- Molecular characteristics of a coxsackievirus A12 strain in Zhejiang of China, 2019.Virology journal · 2022Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
RNA viruses exist as genetically heterogeneous populations due to high mutation rates, and many of these mutations reduce fitness and/or replication speed. However, it is unknown whether mutations can increase replication speed of a virus already well adapted to replication in cultured cells. By sequentially passaging coxsackievirus B3 in cultured cells and collecting the very earliest progeny, we selected for increased replication speed. We found that a single mutation in a viral capsid protein, VP1-F106L, was sufficient for the fast-replication phenotype. Characterization of this mutant revealed quicker genome release during entry compared to wild-type virus, highlighting a previously unappreciated infection barrier. However, this mutation also reduced capsid stability in vitro and reduced replication and pathogenesis in mice. These results reveal a tradeoff between overall replication speed and fitness. Importantly, this approach-selecting for the earliest viral progeny-could be applied to a variety of viral systems and has the potential to reveal unanticipated inefficiencies in viral replication cycles.
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