Evidence map›Paper›PMID 34281265›Full record

ArticleInternational journal of molecular sciences2021

Isolation and Characterization of Barley (

Timo Schlemmer, Patrick Barth, Lisa Weipert, Christian Preußer, Martin Hardt, Anna Möbus, Tobias Busche, Aline Koch

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Review
  5. Article
  6. Diverse plant RNAs coatProceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  7. Review
  8. Article
  9. Article
  10. Review
  11. Beyond Loading: Functions of Plant ARGONAUTE Proteins.International journal of molecular sciences · 2023
    Review
  12. RNA-Based Control of Fungal Pathogens in Plants.International journal of molecular sciences · 2023
    Review
  13. Article
  14. Article
  15. Article
  16. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Timo SchlemmerInstitute of Phytopathology, Centre for BioSystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26, 35392 Giessen, Germany.ORCID 0000-0003-4034-445X
Patrick BarthInstitute of Bioinformatics and Systems Biology, Justus Liebig University, Heinrich-Buff-Ring 58, 35392 Giessen, Germany.ORCID 0000-0002-9156-9694
Lisa WeipertInstitute of Phytopathology, Centre for BioSystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26, 35392 Giessen, Germany.
Christian PreußerInstitute for Tumor Immunology, Center for Tumor Biology and Immunology (ZTI), Philipps University, Hans-Meerwein Strasse 3, 35032 Marburg, Germany.
Martin HardtImaging Unit, Biomedical Research Centre Seltersberg (BFS), Justus Liebig University, Schubertstrasse 81, 35392 Giessen, Germany.
Anna MöbusImaging Unit, Biomedical Research Centre Seltersberg (BFS), Justus Liebig University, Schubertstrasse 81, 35392 Giessen, Germany.
Tobias BuscheCentre for Biotechnology-CeBiTec, Bielefeld University, Universitätsstrasse 25, 33615 Bielefeld, Germany.
Aline KochInstitute of Phytomedicine, University of Hohenheim, Otto-Sander-Strasse 5, 70599 Stuttgart, Germany.

Funding

Bundesministerium für Bildung und Forschung 031A533Deutsche Forschungsgemeinschaft 325443116
6 · The paper itself

Abstract

The demonstration that spray-induced gene silencing (SIGS) can confer strong disease resistance, bypassing the laborious and time-consuming transgenic expression of double-stranded (ds)RNA to induce the gene silencing of pathogenic targets, was ground-breaking. However, future field applications will require fundamental mechanistic knowledge of dsRNA uptake, processing, and transfer. There is increasing evidence that extracellular vesicles (EVs) mediate the transfer of transgene-derived small interfering (si)RNAs in host-induced gene silencing (HIGS) applications. In this study, we establish a protocol for barley EV isolation and assess the possibilities for EVs regarding the translocation of sprayed dsRNA from barley (

Indexed as

Crop ProtectionCytochrome P450 Family 3Disease ResistanceExtracellular VesiclesGene SilencingHordeumHost Microbial InteractionsPlant DiseasesRNA InterferenceRNA, PlantRNA, Small InterferingCytochrome P450 Family 3RNA, PlantRNA, Small InterferingbarleydsRNAextracellular vesiclesFusarium graminearumplant EVRNAiRNA interferenceRNA spraysiRNA

Identifiers

PMID34281265
PMCPMC8268707

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.