Evidence map›Paper›PMID 34280920›Full record

ArticleAudiology & neuro-otology2021

Noise Exposure and Distortion Product Otoacoustic Emission Suprathreshold Amplitudes: A Genome-Wide Association Study.

Joel Lavinsky, Guilherme Kasperbauer, Ricardo F Bento, Aline Mendonça, Juemei Wang, Amanda L Crow, Hooman Allayee, Rick A Friedman

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Audiology & neuro-otology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.6field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 6 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Joel LavinskyPostgraduate Program in Medicine: Surgical Sciences, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
Guilherme KasperbauerPostgraduate Program in Medicine: Surgical Sciences, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
Ricardo F BentoDepartment of Otolaryngology, University of São Paulo, La Jolla, California, USA.
Aline MendonçaPostgraduate Program in Medicine: Surgical Sciences, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
Juemei WangKeck School of Medicine of USC, Los Angeles, California, USA.
Amanda L CrowKeck School of Medicine of USC, Los Angeles, California, USA.
Hooman AllayeeKeck School of Medicine of USC, Los Angeles, California, USA.
Rick A FriedmanUniversity of California, San Diego, California, USA.
Keck Hospital of USC · USUniversidade Federal do Rio Grande do Sul · BRUniversity of California, San Diego · US

Funding

High-Resolution Mapping of Susceptibility Genes for NIHLR01DC010856 · NIDCD · UNIVERSITY OF SOUTHERN CALIFORNIA · PI FRIEDMAN, RICK A · 2011 to 2015
$2.2M
NIDCD NIH HHS R01 DC010856
6 · The paper itself

Abstract

backgroundAlthough several candidate-gene association studies have been conducted to investigate noise-induced hearing loss (NIHL) in humans, most are underpowered, unreplicated, and account for only a fraction of the genetic risk. Mouse genome-wide association studies (GWASs) have revolutionized the field of genetics and have led to the discovery of hundreds of genes involved in complex traits. The hybrid mouse diversity panel (HMDP) is a collection of classic inbred and recombinant inbred strains whose genomes have been either genotyped at high resolution or sequenced. To further investigate the genetics of NIHL, we report the first GWAS based on distortion product otoacoustic emission (DPOAE) measurements and the HMDP.

methodsA total of 102 strains (n = 635) from the HMDP were evaluated based on DPOAE suprathreshold amplitudes before and after noise exposure. DPOAE amplitude variation was set at 60 and 70 dB SPL of the primary tones for each frequency separately (8, 11.3, 16, 22.6, and 32 kHz). These values provided an indirect assessment of outer hair cell integrity. Six-week-old mice were exposed for 2 h to 10 kHz octave-band noise at 108 dB SPL. To perform local expression quantitative trait locus (eQTL) analysis, gene expression microarray profiles were generated using cochlear RNA from 64 hybrid mouse strains (n = 3 arrays per strain).

resultsSeveral new loci were identified and positional candidate-genes associated with NIHL were prioritized, especially after noise exposure (1 locus at baseline and 5 loci after exposure). A total of 35 candidate genes in these 6 loci were identified with at least 1 probe whose expression was regulated by a significant cis-eQTL in the cochlea. After careful analysis of the candidate genes based on cochlear gene expression, 2 candidate genes were prioritized: Eya1 (baseline) and Efr3a (post-exposure). DISCUSSION AND

conclusionFor the first time, an association analysis with correction for population structure was used to map several loci for hearing traits in inbred strains of mice based on DPOAE suprathreshold amplitudes before and after noise exposure. Our results identified a number of novel loci and candidate genes for susceptibility to NIHL, especially the Eya1 and Efr3a genes. Our findings validate the power of the HMDP for detecting NIHL susceptibility genes.

Indexed as

Genome-Wide Association StudyHearing Loss, Noise-InducedAnimalsAuditory ThresholdCochleaMiceNoiseOtoacoustic Emissions, SpontaneousAnimal modelsDistortion product otoacoustic emissionGenome-wide association studyNoiseNoise-induced hearing loss

Identifiers

PMID34280920
PMCPMC8612944
OpenAlexW3186517733

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.