ArticleJournal of insect science (Online)2021
Comparison of Pollen Grain Treatments Without Mechanical Fracturation Prior to Protein Quantification.
Article in Journal of insect science (Online), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 6 citations in OpenAlex.
- A guide to sunflowers: floral resource nutrition for bee health and key pollination syndromes.Frontiers in plant science · 2025Review
- Determination of Nutritional and Biochemical Composition of SelectedBioMed research international · 2023Article
- Assessing pollen nutrient content: a unifying approach for the study of bee nutritional ecology.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Article
- Mass Spectrometry and Computer Simulation Predict the Interactions of AGPS and HNRNPK in Glioma.BioMed research international · 2021Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Protein and amino acids in pollen are important nutritional components for larval development in several insect species, especially in Apoidea. The Bradford assay is a widely used method to measure relative protein content of pollen, which can shed light on pollen quality and consequences to fitness. Prior to using the Bradford assay, protein must be released from pollen grains, often using a mixture of chemical and mechanical fracturation methods. In this study, we tested the efficacy of protein extraction without using mechanical fracturation. We used pollen collected by the solitary bee Osmia lignaria Say to compare two known buffers associated with pollen protein analysis: phosphate-buffered saline and sodium hydroxide, and deionized water, and with different pollen weights from which we quantified protein using the Bradford assay. While all buffers and deionized water were useful in releasing protein from pollen grains collected by O. lignaria, the use of sodium hydroxide resulted in significantly higher protein quantification across all pollen weights. This methodological study can inform future studies of pollen nutrition in pollen-foraging species.
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Registered trials
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