Evidence map›Paper›PMID 34280125›Full record

ArticleEndocrine-related cancer2021

Whole-genome sequencing of single circulating tumor cells from neuroendocrine neoplasms.

Alexa Childs, Christopher D Steele, Clare Vesely, Francesca M Rizzo, Leah Ensell, Helen Lowe, Pawan Dhami, Heli Vaikkinen, Tu Vinh Luong, Lucia Conde and 7 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Endocrine-related cancer, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 14 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 3 institutions in 1 country.

Alexa ChildsUCL Cancer Institute, University College London, London, UK.
Christopher D SteeleUCL Cancer Institute, University College London, London, UK.
Clare VeselyUCL Cancer Institute, University College London, London, UK.
Francesca M RizzoUCL Cancer Institute, University College London, London, UK.
Leah EnsellUCL Cancer Institute, University College London, London, UK.
Helen LoweUCL Cancer Institute, University College London, London, UK.
Pawan DhamiUCL Cancer Institute, University College London, London, UK.
Heli VaikkinenUCL Cancer Institute, University College London, London, UK.
Tu Vinh LuongDepartment of Histopathology, Royal Free London NHS Foundation Trust, London, UK.
Lucia CondeUCL Cancer Institute, University College London, London, UK.
Javier HerreroUCL Cancer Institute, University College London, London, UK.
Martyn CaplinDepartment of Gastroenterology, Royal Free London NHS Foundation Trust, London, UK.
Christos ToumpanakisDepartment of Gastroenterology, Royal Free London NHS Foundation Trust, London, UK.
Christina ThirlwellUCL Cancer Institute, University College London, London, UK.
John A HartleyUCL Cancer Institute, University College London, London, UK.
Nischalan PillayResearch Department of Pathology, Cancer Institute, University College London, London, UK.
Tim MeyerUCL Cancer Institute, University College London, London, UK.
University College London · GBCRUK Lung Cancer Centre of Excellence · GBRoyal Free London NHS Foundation Trust · GB

Funding

Cancer Research UK C12125/A15576Department of HealthMedical Research Council MR/M009033/1
6 · The paper itself

Abstract

Single-cell profiling of circulating tumor cells (CTCs) as part of a minimally invasive liquid biopsy presents an opportunity to characterize and monitor tumor heterogeneity and evolution in individual patients. In this study, we aimed to compare single-cell copy number variation (CNV) data with tissue and define the degree of intra- and inter-patient genomic heterogeneity. We performed next-generation sequencing (NGS) whole-genome CNV analysis of 125 single CTCs derived from seven patients with neuroendocrine neoplasms (NEN) alongside matched white blood cells (WBC), formalin-fixed paraffin-embedded (FFPE), and fresh frozen (FF) samples. CTC CNV profiling demonstrated recurrent chromosomal alterations in previously reported NEN copy number hotspots, including the prognostically relevant loss of chromosome 18. Unsupervised hierarchical clustering revealed CTCs with distinct clonal lineages as well as significant intra- and inter-patient genomic heterogeneity, including subclonal alterations not detectable by bulk analysis and previously unreported in NEN. Notably, we also demonstrated the presence of genomically distinct CTCs according to the enrichment strategy utilized (EpCAM-dependent vs size-based). This work has significant implications for the identification of therapeutic targets, tracking of evolutionary change, and the implementation of CTC-biomarkers in cancer.

Indexed as

Neoplastic Cells, CirculatingNeuroendocrine TumorsBiomarkers, TumorDNA Copy Number VariationsGenomicsHumansWhole Genome SequencingBiomarkers, Tumorcirculating tumor cellscopy number variationneuroendocrine tumorssingle cellwhole-genome sequencing

Identifiers

PMID34280125
PMCPMC8428071
OpenAlexW3183649865

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.