ArticleiScience2021
Development of a gene-deleted live attenuated candidate vaccine against fish virus (ISKNV) with low pathogenicity and high protection.
Article in iScience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 36 citations in OpenAlex.
- STING single amino acid polymorphisms modulate iridovirus immune evasion and pathogenicity spectrum.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- The Role of Gut Microbiota in Fish Viral Infection: Mechanisms and Microbiota-Targeted Interventions.Aquaculture nutrition · 2026Review
- RNF122 targets STING for ubiquitination at residues K95, K117, and K155 to regulate antiviral responses in a teleost fish.Zoological research · 2025Article
- Evaluation of the Multivalent Immunoprotective Effects of Protein, DNA, and IgY Vaccines AgainstInternational journal of molecular sciences · 2025Article
- Effects of Microorganisms in Fish Aquaculture from a Sustainable Approach: A Review.Microorganisms · 2025Review
- Article
- Bacterial diseases in Siniperca chuatsi: status and therapeutic strategies.Veterinary research communications · 2024Review
- Evaluation of a Low-Temperature Immersion Immunization Strategy for the Infectious Spleen and Kidney Necrosis VirusVaccines · 2024Article
- Mandarin fish von Hippel-Lindau protein regulates the NF-κB signaling pathway via interaction with IκB to promote fish ranavirus replication.Zoological research · 2024Article
- Genome-wide identification, characterization, and expression analysis of the transient receptor potential gene family in mandarin fish Siniperca chuatsi.BMC genomics · 2024Article
- Fish Iridoviridae: infection, vaccination and immune response.Veterinary research · 2024Review
- A Potential Nervous Necrosis Virus (NNV) Live Vaccine for Sole Obtained by Genomic Modification.Animals : an open access journal from MDPI · 2024Article
- Hypermethylated genome of a fish vertebrate iridovirus ISKNV plays important roles in viral infection.Communications biology · 2024Article
- Ring finger protein 5 mediates STING degradation through ubiquitinating K135 and K155 in a teleost fish.Frontiers in immunology · 2024Article
- Low-temperature immunization attenuates the residual virulence ofJournal of virology · 2023Article
- Oxygen-Sensing Protein Cysteamine Dioxygenase from Mandarin Fish Involved in the Arg/N-Degron Pathway andViruses · 2023Article
- A Safe and Efficient Double-Gene-Deleted Live Attenuated Immersion Vaccine to Prevent the Disease Caused by the Infectious Spleen and Kidney Necrosis Virus.Journal of virology · 2023Article
- Interaction of Teleost Fish TRPV4 with DEAD Box RNA Helicase 1 Regulates Iridovirus Replication.Journal of virology · 2023Article
- Article
- Review
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aquaculture provides important food, nutrition, and income sources for humans. However, aquaculture industry is seriously threatened by viral diseases. Infectious spleen and kidney necrosis virus (ISKNV) disease causes high mortality and economic losses to the fish culture industry in Asia and has been listed as a certifiable disease by the International Epizootic Office. Vaccine development is urgent to control this disease. Here, a gene-deleted live attenuated candidate vaccine (ΔORF022L) against ISKNV with low pathogenicity and high protection was developed. ΔORF022L replicated well in mandarin fish fry-1 cells and showed similar structure with wild-type ISKNV. However, the pathogenicity was significantly lower as 98% of the mandarin fish infected with ΔORF022L survived, whereas all those infected with wild-type ISKNV died. Of importance, 100% of the ΔORF022L-infected fish survived the ISKNV challenge. ΔORF022L induced anti-ISKNV specific antibody response and upregulation of immune-related genes. This work could be beneficial to the control of fish diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.