Evidence map›Paper›PMID 34277874›Full record

ArticleReports of biochemistry & molecular biology2021

Downregulation of Stemness Genes and Induction of Necrosis in Rat LA7 Cancer Stem Cells Induced Tumors Treated with Starved Fibroblasts Culture Supernatant.

Roghayeh Pourbagher, Hossein Ghorbani, Haleh Akhavan-Niaki, Seyed Gholam Ali Jorsaraei, Sadegh Fattahi, Sahar Ghooran, Zeinab Abedian, Masoumeh Ghasemi, Fatemeh Saeedi, Negar Jafari and 2 more

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In one paragraph

Article in Reports of biochemistry & molecular biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Nutrients · 2022
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 2 countries.

Roghayeh PourbagherStudent Research Committee, Babol University of Medical Sciences, Babol, Iran.
Hossein GhorbaniDepartment of Pathology, Rohani Hospital, Babol University of Medical Sciences, Babol, Iran.
Haleh Akhavan-NiakiCellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
Seyed Gholam Ali JorsaraeiFatemeh Zahra Infertility and Reproductive Health Research Centre, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
Sadegh FattahiCellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
Sahar GhooranDepartment of Pathology, Rohani Hospital, Babol University of Medical Sciences, Babol, Iran.
Zeinab AbedianStudent Research Committee, Babol University of Medical Sciences, Babol, Iran.
Masoumeh GhasemiCellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
Fatemeh SaeediStudent Research Committee, Babol University of Medical Sciences, Babol, Iran.
Negar JafariStudent Research Committee, Babol University of Medical Sciences, Babol, Iran.
Behnam KalaliInstitute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, Munich, Germany.
Amrollah MostafazadehCellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
Babol University of Medical Sciences · IRInstitute of Medical Microbiology and Hygiene · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundStem cell differentiation therapy is a promising strategy in cancer treatment. we show that protein cocktail prepared from serum starved fibroblasts has therapeutic potential based on this strategy.

methodsThe condition medium was prepared from foreskin isolated fibroblasts and analyzed by Liquid chromatography electrospray ionization mass spectrometry-mass spectrometry (LC-ESI-MS/MS). LA7 mammary gland cancer stem cells originated tumors were induced in Sprague Dawley rats. The rats treated subcutaneously with DMEM (group A), condition medium (group B), or normal saline (group C) once daily for 7 days. Then the tumors were removed and divided into the two parts, one part was used to quantify gene expression by stem-loop RT-qPCR assay and the other part was used for Hematoxylin & Eosin (H & E), Giemsa, and immunohistochemistry (IHC) staining.

resultsAll induced tumors appeared as sarcomatoid carcinoma (SC). Immunohistochemistry staining confirmed this conclusion by recognizing the tumor as Ki67

conclusionThis study showed that the substances released from starved human fibroblasts were able to down-regulate the stemness-related genes and induce necrosis in LA7 derived tumors.

Indexed as

Breast cancerCancer Stem cellsCell differentiationFibroblastsGene expression

Identifiers

PMID34277874
PMCPMC8279721
OpenAlexW3165160180

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.