Evidence map›Paper›PMID 34275894›Full record

ArticleCancer biomarkers : section A of Disease markers2021

The miRNA content of circulating exosomes in DLBCL patients and in vitro influence of DLBCL-derived exosomes on miRNA expression of healthy B-cells from peripheral blood.

Vildan Caner, Gokhan Ozan Cetin, Sibel Hacioglu, Ikbal Cansu Baris, Emre Tepeli, Nilay Sen Turk, Gulseren Bagci, Kanay Yararbas, Gulsum Cagliyan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer biomarkers : section A of Disease markers, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 2 syntheses or guidelines pooled it.

  1. Pooled it
  2. Pooled it
  3. Review
  4. Article
  5. Article
  6. Article
  7. Review
  8. Review
  9. Review
  10. Review
  11. Review
  12. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Vildan CanerDepartment of Medical Genetics, School of Medicine, Pamukkale University, Denizli, Turkey.
Gokhan Ozan CetinDepartment of Medical Genetics, School of Medicine, Pamukkale University, Denizli, Turkey.
Sibel HaciogluDepartment of Hematology, School of Medicine, Pamukkale University, Denizli, Turkey.
Ikbal Cansu BarisDepartment of Medical Biology, School of Medicine, Pamukkale University, Denizli, Turkey.
Emre TepeliDepartment of Medical Genetics, School of Medicine, Pamukkale University, Denizli, Turkey.
Nilay Sen TurkDepartment of Medical Pathology, School of Medicine, Pamukkale University, Denizli, Turkey.
Gulseren BagciDepartment of Medical Genetics, School of Medicine, Pamukkale University, Denizli, Turkey.
Kanay YararbasDepartment of Medical Genetics, School of Medicine, Acıbadem Mehmet Ali Aydınlar University, Istanbul, Turkey.
Gulsum CagliyanDepartment of Hematology, School of Medicine, Pamukkale University, Denizli, Turkey.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundDue to the heterogeneous nature of Diffuse Large B-cell Lymphoma (DLBCL), the mechanisms underlying tumor development and progression have not yet been fully elucidated.

objectiveThis study aimed to compare the characteristics of plasma exosomes of DLBCL patients and healthy individuals and to evaluate the exosomal interactions between DLBCL cell lines and normal B-cells.

methodsExosome isolation was performed using an ultracentrifugation-based protocol from plasma of 20 patients with DLBCL and 20 controls. The expression of miRNAs from exosome samples was analyzed using a miRNA expression microarray. The presence of exosome-mediated communication between the lymphoma cells and normal B-cells was determined by the co-culture model.

resultsA significant increase in plasma exosome concentrations of DLBCL patients was observed. There was also a significant decrease in the expression of 33 miRNAs in plasma exosomes of DLBCL patients. It was determined that normal B-cells internalize DLBCL-derived exosomes and then miRNA expression differences observed in normal B-cells are specific to lymphoma-subtypes.

conclusionsMiR-3960, miR-6089 and miR-939-5p can be used as the miRNA signature in DLBCL diagnosis. We suppose that the exosomes changed the molecular signature of the target cells depending on the genomic characterization of the lymphoma cells they have originated.

Indexed as

AdolescentAdultAgedAged, 80 and overB-Lymphocytes, RegulatoryCase-Control StudiesCell Line, TumorExosomesHumansLymphoma, Large B-Cell, DiffuseMicroRNAsMiddle AgedYoung AdultMicroRNAsB-cellscellular uptakeDiffuse large B-cell lymphomaexosomesmiRNA

Identifiers

PMID34275894
PMCPMC12500204

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.