ArticlePlant molecular biology2021
Root engineering in maize by increasing cytokinin degradation causes enhanced root growth and leaf mineral enrichment.
Article in Plant molecular biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Root-specific cytokinin catabolism promotes Nicotiana root growth and resilience to drought stress.Planta · 2026Article
- PcMYBs responded to 6-BA to regulate PcCKXs to promote germination of primary rhizome buds of Polygonatum cyrtonema Hua.BMC plant biology · 2025Article
- Genome-wide investigation of cytokinin oxidase/dehydrogenase (CKX) family genes in Brassica juncea with an emphasis on yield-influencing CKX.Scientific reports · 2025Article
- The recent genetic modification techniques for improve soil conservation, nutrient uptake and utilization.GM crops & food · 2024Review
- Cytokinin: From autoclaved DNA to two-component signaling.The Plant cell · 2024Review
- Characterization and genetic differences analysis in adventitious roots development of 38 Populus germplasm resources.Plant molecular biology · 2024Article
- 14-3-3 Proteins and the Plasma Membrane HPlants (Basel, Switzerland) · 2023Article
- Genome-Wide Identification and Abiotic-Stress-Responsive Expression ofPlants (Basel, Switzerland) · 2023Article
- Novel insights into maize (Zea mays) development and organogenesis for agricultural optimization.Planta · 2023Review
- Modifications of Phytohormone Metabolism Aimed at Stimulation of Plant Growth, Improving Their Productivity and Tolerance to Abiotic and Biotic Stress Factors.Plants (Basel, Switzerland) · 2022Review
- Maize Seedling Growth and Hormone Response Assays Using the Rolled Towel Method.Current protocols · 2022Article
- GWAS and Transcriptome Analysis Reveal Key Genes Affecting Root Growth under Low Nitrogen Supply in Maize.Genes · 2022Article
- The LONELY GUY gene family: from mosses to wheat, the key to the formation of active cytokinins in plants.Plant biotechnology journal · 2022Review
- Guvermectin, a novel plant growth regulator, can promote the growth and high temperature tolerance of maize.Frontiers in plant science · 2022Article
- Transcriptomics and metabolomics changes triggered by exogenous 6-benzylaminopurine in relieving epicotyl dormancy ofFrontiers in plant science · 2022Article
- Biological Parts for Plant Biodesign to Enhance Land-Based Carbon Dioxide Removal.Biodesign research · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageRoot-specific expression of a cytokinin-degrading CKX gene in maize roots causes formation of a larger root system leading to higher element content in shoot organs. The size and architecture of the root system is functionally relevant for the access to water and soil nutrients. A great number of mostly unknown genes are involved in regulating root architecture complicating targeted breeding of plants with a larger root system. Here, we have explored whether root-specific degradation of the hormone cytokinin, which is a negative regulator of root growth, can be used to genetically engineer maize (Zea mays L.) plants with a larger root system. Root-specific expression of a CYTOKININ OXIDASE/DEHYDROGENASE (CKX) gene of Arabidopsis caused the formation of up to 46% more root dry weight while shoot growth of these transgenic lines was similar as in non-transgenic control plants. The concentration of several elements, in particular of those with low soil mobility (K, P, Mo, Zn), was increased in leaves of transgenic lines. In kernels, the changes in concentration of most elements were less pronounced, but the concentrations of Cu, Mn and Zn were significantly increased in at least one of the three independent lines. Our data illustrate the potential of an increased root system as part of efforts towards achieving biofortification. Taken together, this work has shown that root-specific expression of a CKX gene can be used to engineer the root system of maize and alter shoot element composition.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.