ArticleJournal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)2021
Evaluation of in vitro activity of copper gluconate against SARS-CoV-2 using confocal microscopy-based high content screening.
Article in Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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12 citing papers in PubMed, 1 synthesis or guideline pooled it, 22 citations in OpenAlex.
- Efficacy of Mouth Rinses and Nasal Spray in the Inactivation of SARS-CoV-2: A Systematic Review and Meta-Analysis ofInternational journal of environmental research and public health · 2022Pooled it
- Contrastive learning of dynamic processing body formation reveals undefined mechanisms of approved compounds.iScience · 2026Article
- Zinc in Soil-Plant-Human Systems: A Synthesis of Its Fate, Essential Roles, and Health Implications.Scientifica · 2026Review
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- UiO-66 nanoparticles combat influenza A virus in mice by activating the RIG-I-like receptor signaling pathway.Journal of nanobiotechnology · 2024Article
- Disinfection and decontamination in the context of SARS-CoV-2-specific data.Journal of medical virology · 2022Review
- High-content screening of active components of Traditional Chinese Medicine inhibiting TGF-β-induced cell EMT.Heliyon · 2022Article
- Inhibition of SARS-CoV-2 replication by zinc gluconate in combination with hinokitiol.Journal of inorganic biochemistry · 2022Article
- Inhibition of Respiratory RNA Viruses by a Composition of Ionophoric Polyphenols with Metal Ions.Pharmaceuticals (Basel, Switzerland) · 2022Article
- Copper acetate aerosols: A possible tool complementary to vaccination in fight against SARS-CoV-2 and variants replication.Medical hypotheses · 2022Article
- The Effect of Circulating Zinc, Selenium, Copper and Vitamin KNutrients · 2022Article
- Potential molecular mechanisms of zinc- and copper-mediated antiviral activity on COVID-19.Nutrition research (New York, N.Y.) · 2021Review
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
contextSevere Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) that emerged late in 2019 is the etiologic agent of coronavirus disease 2019 (Covid-19). There is an urgent need to develop curative and preventive therapeutics to limit the current pandemic and to prevent the re-emergence of Covid-19. This study aimed to assess the in vitro activity of copper gluconate against SARS-CoV-2.
methodsVero E6 cells were cultured with or without copper gluconate 18-24 hours before infection. Cells were infected with a recombinant GFP expressing SARS-CoV-2. Cells were infected with a recombinant GFP expressing SARS-CoV-2. Infected cells were incubated in fresh medium containing varying concentration of copper gluconate (supplemented with bovine serum albumin or not) for an additional 48 -h period. The infection level was measured by the confocal microscopy-based high content screening method. The cell viability in presence of copper gluconate was assessed by XTT and propidium iodide assays.
resultsThe viability of Vero E6 cells exposed to copper gluconate up to 200 μM was found to be similar to that of unexposed cells, but it dropped below 70 % with 400 μM of this agent after 72 h of continuous exposure. The infection rate was 23.8 %, 18.9 %, 20.6 %, 6.9 %, 5.3 % and 5.2 % in cells treated prior infection with 0, 2, 10, 25, 50 and 100 μM of copper gluconate respectively. As compared to untreated cells, the number of infected cells was reduced by 71 %, 77 %, and 78 % with 25, 50, and 100 μM of copper gluconate respectively (p < 0.05). In cells treated only post-infection, the rate of infection dropped by 73 % with 100 μM of copper gluconate (p < 0.05). However, the antiviral activity of copper gluconate was abolished by the addition of bovine serum albumin.
conclusionCopper gluconate was found to mitigate SARS-CoV-2 infection in Vero E6 cells but this effect was abolished by albumin, which suggests that copper will not retain its activity in serum. Furthers studies are needed to investigate whether copper gluconate could be of benefit in mucosal administration such as mouthwash, nasal spray or aerosols.
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