ArticleBMC plant biology2021
A tubby-like protein CsTLP8 acts in the ABA signaling pathway and negatively regulates osmotic stresses tolerance during seed germination.
Article in BMC plant biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- Population genomics reveals association of transposable elements variants with climatic adaptation in wild Amur grape.Nature communications · 2026Article
- Characterization and Expression Analysis of theInternational journal of molecular sciences · 2025Article
- A tubby-like protein, MdTLP7 enhances drought and salt stresses tolerance of Malus domestica.BMC plant biology · 2025Article
- Deciphering the mechanism of E3 ubiquitin ligases in plant responses to abiotic and biotic stresses and perspectives on PROTACs for crop resistance.Plant biotechnology journal · 2024Review
- Molecular analyses of the tubby-like protein gene family and their response to salt and high temperature in the foxtail millet (Setaria italica).Functional & integrative genomics · 2024Article
- Research Advancements in Salt Tolerance of Cucurbitaceae: From Salt Response to Molecular Mechanisms.International journal of molecular sciences · 2024Review
- Comprehensive Genome-Wide Natural Variation and Expression Analysis of Tubby-like Proteins Gene Family inPlants (Basel, Switzerland) · 2024Article
- Improvement of plant tolerance to drought stress by cotton tubby-like protein 30 through stomatal movement regulation.Journal of advanced research · 2022Article
- Genome-Wide Identification, Characterization, and Expression Analysis of Tubby-like Protein (TLP) Gene Family Members in Woodland Strawberry (International journal of molecular sciences · 2022Article
- Response ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Serial-Omics and Molecular Function Study Provide Novel Insight into Cucumber Variety Improvement.Plants (Basel, Switzerland) · 2022Review
- Genome-wide identification and comprehensive analysis of tubby-like protein gene family in multiple crops.Frontiers in plant science · 2022Article
- Comprehensive Profiling of Tubby-Like Proteins in Soybean and Roles of theFrontiers in plant science · 2022Article
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTLPs (Tubby-like proteins) are widespread in eukaryotes and highly conserved in plants and animals. TLP is involved in many biological processes, such as growth, development, biotic and abiotic stress responses, while the underlying molecular mechanism remains largely unknown. In this paper we characterized the biological function of cucumber (Cucumis sativus L.) Tubby-like protein 8 (CsTLP8) in Arabidopsis.
resultsIn cucumber, the expression of the tubby-like protein CsTLP8 was induced by NaCl treatment, but reduced by PEG (Polyethylene Glycol) and ABA (Abscisic Acid) treatment. Subcellular localization and transcriptional activation activity analysis revealed that CsTLP8 possessed two characteristics of classical transcription factors: nuclear localization and trans-activation activity. Yeast two-hybrid assay revealed interactions of CsTLP8 with CsSKP1a and CsSKP1c, suggesting that CsTLP8 might function as a subunit of E3 ubiquitin ligase. The growth activity of yeast with ectopically expressed CsTLP8 was lower than the control under NaCl and mannitol treatments. Under osmotic and salt stresses, overexpression of CsTLP8 inhibited seed germination and the growth of Arabidopsis seedlings, increased the content of MDA (Malondialdehyde), and decreased the activities of SOD (Superoxide Dismutase), POD (Peroxidase) and CAT (Catalase) in Arabidopsis seedlings. Overexpression of CsTLP8 also increased the sensitivity to ABA during seed germination and ABA-mediated stomatal closure.
conclusionUnder osmotic stress, CsTLP8 might inhibit seed germination and seedling growth by affecting antioxidant enzymes activities. CsTLP8 acts as a negative regulator in osmotic stress and its effects may be related to ABA.
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