Evidence map›Paper›PMID 34272844›Full record

ArticleThe Journal of clinical endocrinology and metabolism2021

Ex Vivo Intact Tissue Analysis Reveals Alternative Calcium-sensing Behaviors in Parathyroid Adenomas.

James Koh, Run Zhang, Sanziana Roman, Quan-Yang Duh, Jessica Gosnell, Wen Shen, Insoo Suh, Julie A Sosa

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of clinical endocrinology and metabolism, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

James KohEndocrine Neoplasia Laboratory, Department of Surgery, University of California at San Francisco, San Francisco, CA, USA.ORCID 0000-0001-6853-8606
Run ZhangEndocrine Neoplasia Laboratory, Department of Surgery, University of California at San Francisco, San Francisco, CA, USA.ORCID 0000-0003-4876-810X
Sanziana RomanDepartment of Surgery, University of California at San Francisco, San Francisco, CA, USA.ORCID 0000-0002-6911-2508
Quan-Yang DuhDepartment of Surgery, University of California at San Francisco, San Francisco, CA, USA.ORCID 0000-0002-7947-4393
Jessica GosnellDepartment of Surgery, University of California at San Francisco, San Francisco, CA, USA.ORCID 0000-0002-0640-5418
Wen ShenDepartment of Surgery, University of California at San Francisco, San Francisco, CA, USA.ORCID 0000-0001-7531-1450
Insoo SuhDepartment of Surgery, NYU Langone Health, New York, NY, USA.ORCID 0000-0001-6911-2324
Julie A SosaEndocrine Neoplasia Laboratory, Department of Surgery, University of California at San Francisco, San Francisco, CA, USA.ORCID 0000-0002-3356-7316

Funding

Parathyroid Tumor Clonal Status as a Biomarker in Primary HyperparathyroidismR01CA228399 · NCI · WASHINGTON UNIVERSITY · PI OLSON, JOHN A. · 2019 to 2023
$3.2M
Regulation of Parathyroid Functions By G-Protein Coupled ReceptorsR01DK122259 · NIDDK · NORTHERN CALIFORNIA INSTITUTE/RES/EDU · PI CHANG, WENHAN, VILARDAGA, JEAN-PIERRE · 2019 to 2023
$2.2M
Regulation of parathyroid function by the amyloid precursor proteinR21AG070721 · NIA · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI CHANG, WENHAN, KOH, JAMES · 2021 to 2022
$444k
NCI NIH HHS R01 CA228399NIA NIH HHS R21 AG070721NIDDK NIH HHS R01 DK122259NIH HHS 1 R01 CA228399-02A1
6 · The paper itself

Abstract

contextThe biochemical basis for clinical variability in primary hyperparathyroidism (PHPT) is poorly understood.

objectiveThis study aimed to define parathyroid tumor biochemical properties associated with calcium-sensing failure in PHPT patients, and to relate differences in these profiles to variations in clinical presentation.

methodsPreoperative clinical data from a sequential series of 39 patients undergoing surgery for PHPT at an endocrine surgery referral center in a large, public university hospital were evaluated for correlation to parathyroid tumor biochemical behavior. An intact tissue, ex vivo interrogative assay was employed to evaluate the calcium-sensing capacity of parathyroid adenomas relative to normal donor glands. Tumors were functionally classified based on calcium dose-response curve profiles, and clinical parameters were compared among the respective classes. Changes in the relative expression of 3 key components in the calcium/parathyroid hormone (PTH) signaling axis-CASR, RGS5, and RCAN1-were evaluated as potential mechanisms for calcium-sensing failure.

resultsParathyroid adenomas grouped into 3 distinct functional classes. Tumors with diminished calcium sensitivity were the most common (18 of 39) and were strongly associated with reduced bone mineral density (P = 0.0009). Tumors with no calcium-sensing deficit (11 of 39) were associated with higher preoperative PTH (P = 0.036). A third group (6/39) displayed a nonsigmoid calcium/PTH response curve; 4 of these 6 tumors expressed elevated RCAN1.

conclusionCalcium-sensing capacity varies among parathyroid tumors but downregulation of the calcium-sensing receptor (CASR) is not an obligate underlying mechanism. Differences in tumor calcium responsiveness may contribute to variations in PHPT clinical presentation.

Indexed as

AdenomaAgedBiomarkersCalciumCase-Control StudiesDNA-Binding ProteinsFemaleFollow-Up StudiesHumansHyperparathyroidism, PrimaryMaleMiddle AgedMuscle ProteinsParathyroid HormoneParathyroid NeoplasmsPrognosisBiomarkersCalciumDNA-Binding ProteinsMuscle ProteinsParathyroid HormoneRCAN1 protein, humanReceptors, Calcium-SensingRGS5 protein, humanRGS Proteinscalcium sensingparathyroid adenomaprimary hyperparathyroidism

Identifiers

PMID34272844
PMCPMC8530711

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.