Evidence map›Paper›PMID 34271099›Full record

ArticleThe Journal of nutritional biochemistry2021

Gut probiotic Lactobacillus rhamnosus attenuates PDE4B-mediated interleukin-6 induced by SARS-CoV-2 membrane glycoprotein.

Minh Tan Pham, Albert Jackson Yang, Ming-Shan Kao, Uuganbayar Gankhuyag, Enkhbat Zayabaatar, Shiow-Lian Catherine Jin, Chun-Ming Huang

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of nutritional biochemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.9field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 29 citations in OpenAlex.

  1. Article
  2. Modulatory Roles ofViruses · 2024
    Article
  3. Article
  4. Review
  5. Review
  6. Microorganisms · 2024
    Review
  7. Review
  8. Review
  9. Review
  10. The Beneficial Role of ProbioticFrontiers in immunology · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Minh Tan PhamDepartment of Biomedical Sciences and Engineering, National Central University, Taoyuan, 32001, Taiwan.
Albert Jackson YangDepartment of Biomedical Sciences and Engineering, National Central University, Taoyuan, 32001, Taiwan.
Ming-Shan KaoDepartment of Biomedical Sciences and Engineering, National Central University, Taoyuan, 32001, Taiwan.
Uuganbayar GankhuyagDepartment of Biomedical Sciences and Engineering, National Central University, Taoyuan, 32001, Taiwan.
Enkhbat ZayabaatarDepartment of Biomedical Sciences and Engineering, National Central University, Taoyuan, 32001, Taiwan.
Shiow-Lian Catherine JinDepartment of Life Sciences, National Central University, Taoyuan, 32001, Taiwan.
Chun-Ming HuangDepartment of Biomedical Sciences and Engineering, National Central University, Taoyuan, 32001, Taiwan; Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung, 80708, Taiwan. Electronic address: chunming@ncu.edu.tw.
National Central University · TWKaohsiung Medical University · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Membrane glycoprotein is the most abundant protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), but its role in coronavirus disease 2019 (COVID-19) has not been fully characterized. Mice intranasally inoculated with membrane glycoprotein substantially increased the interleukin (IL)-6, a hallmark of the cytokine storm, in bronchoalveolar lavage fluid (BALF), compared to mice inoculated with green fluorescent protein (GFP). The high level of IL-6 induced by membrane glycoprotein was significantly diminished in phosphodiesterase 4 (PDE4B) knockout mice, demonstrating the essential role of PDE4B in IL-6 signaling. Mycelium fermentation of Lactobacillus rhamnosus (L. rhamnosus) EH8 strain yielded butyric acid, which can down-regulate the PDE4B expression and IL-6 secretion in macrophages. Feeding mice with mycelia increased the relative abundance of commensal L. rhamnosus. Two-week supplementation of mice with L. rhamnosus plus mycelia considerably decreased membrane glycoprotein-induced PDE4B expression and IL-6 secretion. The probiotic activity of L. rhamnosus plus mycelia against membrane glycoprotein was abolished in mice treated with GLPG-0974, an antagonist of free fatty acid receptor 2 (Ffar2). Activation of Ffar2 in the gut-lung axis for down-regulation of the PDE4B-IL-6 signalling may provide targets for development of modalities including probiotics for treatment of the cytokine storm in COVID-19.

Indexed as

AnimalsButyric AcidCell LineCloning, MolecularCoronavirus M ProteinsCyclic Nucleotide Phosphodiesterases, Type 4FemaleFermentationGene Expression RegulationHumansInterleukin-6Lacticaseibacillus rhamnosusMiceMice, Inbred ICRProbioticsReceptors, G-Protein-CoupledButyric AcidCoronavirus M ProteinsCyclic Nucleotide Phosphodiesterases, Type 4Ffar2 protein, mouseInterleukin-6PDE4B protein, mouseReceptors, G-Protein-CoupledCytokine stormIL-6Lactobacillus rhamnosusPDE4BSARS-CoV-2 membrane glycoprotein

Identifiers

PMID34271099
PMCPMC8277543
OpenAlexW3178428051

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.