ArticleMolecular cell2021
CTP regulates membrane-binding activity of the nucleoid occlusion protein Noc.
Article in Molecular cell, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 40 citations in OpenAlex.
- The role of the VirB ligand CTP in the molecular mechanism of transcriptional anti-silencing in Shigella flexneri.Nucleic acids research · 2026Article
- Versatile NTP recognition and domain fusions expand the functional repertoire of the ParB-CTPase fold beyond chromosome segregation.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- How do spherical bacteria regulate cell division?Biochemical Society transactions · 2025Review
- Review
- KorB switching from DNA-sliding clamp to repressor mediates long-range gene silencing in a multi-drug resistance plasmid.Nature microbiology · 2025Article
- Tracking pairwise genomic loci by the ParB-ParS and Noc-NBS systems in living cells.Nucleic acids research · 2024Article
- Macromolecular Crowding, Phase Separation, and Homeostasis in the Orchestration of Bacterial Cellular Functions.Chemical reviews · 2024Review
- Connecting the dots: key insights on ParB for chromosome segregation from single-molecule studies.FEMS microbiology reviews · 2024Review
- The virulence regulator VirB from Shigella flexneri uses a CTP-dependent switch mechanism to activate gene expression.Nature communications · 2024Article
- Clostridioides difficile Sporulation.Advances in experimental medicine and biology · 2024Review
- VirB, a transcriptional activator of virulence in Shigella flexneri, uses CTP as a cofactor.Communications biology · 2023Article
- Cell cycle-dependent organization of a bacterial centromere through multi-layered regulation of the ParABS system.PLoS genetics · 2023Article
- A model industrial workhorse: Bacillus subtilis strain 168 and its genome after a quarter of a century.Microbial biotechnology · 2023Review
- 7-Deazaguanines in DNA: functional and structural elucidation of a DNA modification system.Nucleic acids research · 2023Article
- Bacterial division ring stabilizing ZapA versus destabilizing SlmA modulate FtsZ switching between biomolecular condensates and polymers.Open biology · 2023Article
- Membrane mediated phase separation of the bacterial nucleoid occlusion protein Noc.Scientific reports · 2022Article
- Stochastically multimerized ParB orchestrates DNA assembly as unveiled by single-molecule analysis.Nucleic acids research · 2022Article
- A joint-ParB interface promotes Smc DNA recruitment.Cell reports · 2022Article
- RefZ and Noc Act Synthetically to Prevent Aberrant Divisions during Bacillus subtilis Sporulation.Journal of bacteriology · 2022Article
- Relief of ParB autoinhibition byScience advances · 2021Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
ATP- and GTP-dependent molecular switches are extensively used to control functions of proteins in a wide range of biological processes. However, CTP switches are rarely reported. Here, we report that a nucleoid occlusion protein Noc is a CTPase enzyme whose membrane-binding activity is directly regulated by a CTP switch. In Bacillus subtilis, Noc nucleates on 16 bp NBS sites before associating with neighboring non-specific DNA to form large membrane-associated nucleoprotein complexes to physically occlude assembly of the cell division machinery. By in vitro reconstitution, we show that (1) CTP is required for Noc to form the NBS-dependent nucleoprotein complex, and (2) CTP binding, but not hydrolysis, switches Noc to a membrane-active state. Overall, we suggest that CTP couples membrane-binding activity of Noc to nucleoprotein complex formation to ensure productive recruitment of DNA to the bacterial cell membrane for nucleoid occlusion activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.