ArticleMethods in molecular biology (Clifton, N.J.)2021
Triticale Isolated Microspore Culture for Doubled Haploid Production.
Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- New Epigenetic Modifier Inhibitors Enhance Microspore Embryogenesis in Bread Wheat.Plants (Basel, Switzerland) · 2024Article
- Induction of Triticale (×Life (Basel, Switzerland) · 2023Article
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Authors and funding
2 authors.
Funding
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Abstract
Here, we describe a method of triticale isolated microspore culture for production of doubled haploid plants via androgenesis. We use this method routinely because it is highly efficient and works well on different triticale genotypes. To force microspores into becoming embryogenic, we apply a 21-day cold pretreatment. The shock of cold facilitates redirecting microspores from their predestined pollen developmental program into the androgenesis pathway. Ovaries are included in our culture methods to help with embryogenesis, and the histone deacytelase inhibitor Trichostatin A (TSA) is added to further improve androgenesis and increase our ability to recover green doubled haploid plants.
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