ArticleMethods in molecular biology (Clifton, N.J.)2021
Analysis of Ploidy in Haploids and Doubled Haploids.
Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed.
- Comparative evaluation of maize chromosome pretreatment using cold water and 8-hydroxyquinoline.Planta · 2025Article
- Distinction of chia varieties in vivo and in vitro based on the flow cytometry and rosmarinic acid production.Applied microbiology and biotechnology · 2024Article
- The Nuclear 35S rDNA World in Plant Systematics and Evolution: A Primer of Cautions and Common Misconceptions in Cytogenetic Studies.Frontiers in plant science · 2022Review
- Structure and Methylation of 35S rDNA in AllopolyploidsFrontiers in plant science · 2022Article
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Authors and funding
2 authors.
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Abstract
Determination of the ploidy level is an essential step when trying to produce doubled haploids (DHs) in any species. Each species and method used to produce DHs has its own frequency of DH production, which means that the rest of plants produced stay haploid. Since haploids are of little use for breeding purposes, it is necessary to distinguish them from true DHs. For this, several methodologies are available, including flow cytometry, chromosome counting, chloroplast counting in stomatal guard cells, measurement of stomatal size and length, counting of nucleoli, evaluation of pollen formation and viability, analysis of cell size, and analysis of morphological markers. However, not all of them are equally easy to use, affordable, reliable, reproducible, and resolutive and therefore useful for a particular case. In this chapter, we revise these methods available to assess the ploidy level of plants, discussing their respective advantages and limitations, and provide some troubleshooting tips and hints to help decide which to choose in each case.
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