ReviewMethods in molecular biology (Clifton, N.J.)2021
Overview of In Vitro and In Vivo Doubled Haploid Technologies.
Review in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed.
- Rapid Genetic Stabilisation of Transgenic and Edited Wheat Through Transformation of Immature Haploid Embryos.Plant biotechnology journal · 2026Article
- Defining the Optimal Microspore Developmental Window for Efficient Anther-Derived Somatic Embryogenesis in Rubber Tree (Plants (Basel, Switzerland) · 2026Article
- Association of morphological markers of flower buds and anthers with the pollen developmental stage inFrontiers in plant science · 2026Article
- Unresolved ER stress restricts in vitro plant cell totipotency.Plant cell reports · 2025Article
- Modulation of calcium, callose synthesis, membrane permeability and pectin methyl-esterase activity affect cell wall composition and embryo yield during Brassica napus microspore embryogenesis.Annals of botany · 2025Article
- Haploid Production inBiology · 2025Review
- Genetics, genomics and breeding of fennel.BMC plant biology · 2025Review
- Effects of hormone concentrations on anther cultures and the acquisition of regenerated plants of five awnless triticale genotypes.Plant methods · 2025Article
- PidTools: Algorithm and web tools for crop pedigree identification analysis.Computational and structural biotechnology journal · 2024Article
- Efficient isolated microspore culture protocol for callus induction and plantlet regeneration in japonica rice (Oryza sativa L.).Plant methods · 2024Article
- Calcium levels modulate embryo yield inFrontiers in plant science · 2024Article
- The Highly EmbryogenicPlants (Basel, Switzerland) · 2023Article
- Calcium Dynamics, WUSCHEL Expression and Callose Deposition during Somatic Embryogenesis inPlants (Basel, Switzerland) · 2023Article
- Genetic resources and breeding of maize forFrontiers in plant science · 2023Review
- Calcium dynamics and modulation in carrot somatic embryogenesis.Frontiers in plant science · 2023Article
- Cell Wall Composition and Structure Define the Developmental Fate of Embryogenic Microspores inFrontiers in plant science · 2021Article
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3 authors.
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Abstract
Doubled haploids (DH) have become a powerful tool to assist in different basic research studies, and also in applied research. The principal (but not the only) and routine use of DH by breeding companies is to produce pure lines for hybrid seed production in different crop species. Several decades after the discovery of haploid inducer lines in maize and of anther culture as a method to produce haploid plants from pollen precursors, the biotechnological revolution of the last decades allowed to the development of a variety of approaches to pursue the goal of doubled haploid production. Now, it is possible to produce haploids and DHs in many different species, because when a method does not work properly, there are several others to test. In this chapter, we overview the currently available approaches used to produce haploids and DHs by using methods based on in vitro culture, or involving the in vivo induction of haploid embryo development, or a combination of both.
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