ArticleCurrent microbiology2021
In Vitro and In Silico Inhibition of Staphylococcus aureus Efflux Pump NorA by α-Pinene and Limonene.
Article in Current microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 35 citations in OpenAlex.
- Unraveling the volatile profile and bioactivity of Zhoupigan (Frontiers in nutrition · 2026Article
- Recent insights in the importance of limonene and its biotransformed value added compounds.World journal of microbiology & biotechnology · 2025Review
- Impact of small antral pseudocyst interventions on bone remodeling during sinus floor elevation: a retrospective analysis.BMC oral health · 2025Article
- Preliminary Evaluation of the Synergistic Antibacterial Effects of Selected Commercial Essential Oil Compounds Against Methicillin-ResistantAntibiotics (Basel, Switzerland) · 2025Article
- Global trends and biological activity hotspots of D-limonene in essential oils: a 30-year bibliometric study.Naunyn-Schmiedeberg's archives of pharmacology · 2025Article
- Combating multidrug-resistant (MDR) Staphylococcus aureus infection using terpene and its derivative.World journal of microbiology & biotechnology · 2024Review
- Genomic Comparative of Pseudomonas aeruginosa Small Colony Variant, Mucoid and Non-mucoid Phenotypes Obtained from a Patient with Cystic Fibrosis During Respiratory Exacerbations.Current microbiology · 2024Article
- Antimicrobial Activity of Individual Volatile Compounds from Various Essential Oils.Molecules (Basel, Switzerland) · 2024Article
- Essential Oil Compounds in Combination with Conventional Antibiotics for Dermatology.Molecules (Basel, Switzerland) · 2024Article
- Article
- 3-Substituted Coumarins Inhibit NorA and MepA Efflux Pumps ofAntibiotics (Basel, Switzerland) · 2023Article
- Antibacterial Activity of the Essential Oil ofPlants (Basel, Switzerland) · 2023Article
- Chemical Composition and Antimicrobial Potential of Essential Oil ofPharmaceuticals (Basel, Switzerland) · 2022Article
- Modulation of Drug Resistance by Furanochromones inEvidence-based complementary and alternative medicine : eCAM · 2022Article
- Chalcone Derivatives as Potential Inhibitors of P-Glycoprotein and NorA: AnBioMed research international · 2022Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Since the discovery of the first antibiotics, bacteria have acquired a variety of resistance mechanisms, with efflux pump (EP) being the most prominent mechanism for intracellular targeting drugs. These proteins have become efficient mechanisms of resistance to antibiotics in species such as Staphylococcus aureus and, therefore, have been identified as promising therapeutic targets in antibacterial drug development. Accordingly, evidence suggests that monoterpenes can act as EP inhibitors and can be useful in circumventing bacterial resistance. This study aimed to evaluate the effectiveness of monoterpenes α-pinene and limonene as EP inhibitors against a strain of S. aureus expressing NorA protein. The minimum inhibitory concentration (MIC) against the 1199B strain of S. aureus, which carries genes encoding efflux proteins associated with antibiotic resistance to norfloxacin, was assessed through the broth microdilution method. The results obtained served as a subsidy for the analysis of the NorA pump inhibition with norfloxacin and ethidium bromide. Docking techniques, in silico, were used to evaluate the interaction of monoterpenes with NorA. Both monoterpenes showed no clinically effective antibacterial activity. Nevertheless, these compounds were found to decrease the MICs of ethidium bromide and norfloxacin indicating EP inhibition, which was confirmed by molecular docking analyses. In conclusion, α-pinene and limonene showed promising antibiotic-enhancing properties in S. aureus 1199B strain, indicating that monoterpenes can be used in targeted drug development to combat antibiotic resistance associated with EP expression.
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