Evidence map›Paper›PMID 34268019›Full record

ArticleApplications in plant sciences2021

Single-nucleotide polymorphisms that uniquely identify cultivars of avocado (

Wiebke Kämper, Stephen J Trueman, Jade Cooke, Naga Kasinadhuni, Aaron J Brunton, Steven M Ogbourne

Abstract read
In one paragraph

Article in Applications in plant sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Wiebke KämperFood Security Platform Centre for Planetary Health and Food Security School of Environment and Science Griffith University Nathan Queensland 4111 Australia.ORCID https://orcid.org/0000-0002-8646-4492
Stephen J TruemanFood Security Platform Centre for Planetary Health and Food Security School of Environment and Science Griffith University Nathan Queensland 4111 Australia.ORCID https://orcid.org/0000-0002-7105-7130
Jade CookeAustralian Genome Research Facility Melbourne Victoria 3000 Australia.
Naga KasinadhuniAustralian Genome Research Facility Brisbane Queensland 4072 Australia.
Aaron J BruntonGeneCology Research Centre University of the Sunshine Coast Maroochydore DC Queensland 4558 Australia.ORCID https://orcid.org/0000-0002-6959-0059
Steven M OgbourneGeneCology Research Centre University of the Sunshine Coast Maroochydore DC Queensland 4558 Australia.ORCID https://orcid.org/0000-0001-9812-4367

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

premiseProgeny of avocado (

methodsA double-digest restriction site-associated DNA sequencing (ddRADseq) approach was used to screen 10 avocado cultivars for single-nucleotide polymorphisms (SNPs). The fragments were size selected with Blue Pippin and PCR using universal Illumina primers, and catalog tags were then created with de novo alignment using Stacks software. Catalog tags were tabulated and filtered to identify alleles unique to each cultivar.

resultsA total of 104 million sequences were collected, and 52 homozygous SNPs were identified that uniquely distinguished nine avocado cultivars. The cultivars Carmen Hass and Hass have a strong genetic similarity and no homozygous SNPs distinguishing these cultivars could be identified; therefore, both cultivars were grouped together. DISCUSSION: The resource described here for cultivars of

Indexed as

avocadobreedingddRADSeqgene flowpollination

Identifiers

PMID34268019
PMCPMC8272581

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.