ArticleApplications in plant sciences2021
Algae to angiosperms: Autofluorescence for rapid visualization of plant anatomy among diverse taxa.
Article in Applications in plant sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 27 citations in OpenAlex.
- Long-term preservation strategy for legume root nodule phenotypes coupled with a comprehensive evaluation method.BMC plant biology · 2026Article
- Artificial MicroRNA delivery and gene silencing via extracellular vesicles derived from molecularly modified tobacco.Protoplasma · 2026Article
- The Rapid Anatomics Tool (RAT): A low-cost root anatomical phenotyping platform reveals changes in root anatomy along the root axis.Plant phenomics (Washington, D.C.) · 2026Article
- Autofluorescence and Metabotyping of Soybean Varieties Using Confocal Laser Microscopy and High-Resolution Mass Spectrometric Approaches.Plants (Basel, Switzerland) · 2025Article
- Autofluorescence-based tissue characterization enhances clinical prospects of light-sheet-microscopy.Scientific reports · 2024Article
- Mutation mapping of a variegated EMS tomato reveals an FtsH-like protein precursor potentially causing patches of four phenotype classes in the leaves with distinctive internal morphology.BMC plant biology · 2024Article
- Stress phenotyping analysis leveraging autofluorescence image sequences with machine learning.Frontiers in plant science · 2024Article
- How to explore what is hidden? A review of techniques for vascular tissue expression profile analysis.Plant methods · 2023Review
- Improving the Autofluorescence ofPolymers · 2023Article
- Simultaneous detection of miRNA and mRNA at the single-cell level in plant tissues.Plant biotechnology journal · 2023Article
- Autofluorescence-Based Investigation of Spatial Distribution of Phenolic Compounds in Soybeans Using Confocal Laser Microscopy and a High-Resolution Mass Spectrometric Approach.Molecules (Basel, Switzerland) · 2022Article
- Algae to angiosperms: Autofluorescence for rapid visualization of plant anatomy among diverse taxa.Applications in plant sciences · 2021Article
- High-speed video and plant ultrastructure define mechanisms of gametophyte dispersal.Applications in plant sciencesArticle
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
premiseFluorescence microscopy is an effective tool for viewing plant internal anatomy. However, using fluorescent antibodies or labels hinders throughput. We present a minimal protocol that takes advantage of inherent autofluorescence and aldehyde-induced fluorescence in plant cellular and subcellular structures to markedly increase throughput in cellular and ultrastructural visualization. METHODS AND
resultsTwelve species distributed across the plant phylogeny were each subjected to five fixative treatments: 1% paraformaldehyde and 2% glutaraldehyde, 2% paraformaldehyde, 2% glutaraldehyde, formalin-acid-alcohol (FAA), and 70% ethanol. Samples were prepared by embedding and mechanically sectioning or via whole mount. A confocal laser scanning system was used to collect micrographs. We evaluated and compared fixative influence on sample structural preservation and tissue autofluorescence.
conclusionsFormaldehyde fixation of Viridiplantae taxa samples generates useful structural data while requiring no additional histological staining or clearing. In addition, a fluorescence-capable microscope is the only specialized equipment required for image acquisition. The minimal protocol developed in this experiment enables high-throughput sample processing by eliminating the need for multi-day preparations.
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