ArticleNature microbiology2021
Structural and functional characterization of the intracellular filament-forming nitrite oxidoreductase multiprotein complex.
Article in Nature microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 77 citations in OpenAlex.
- Photoholes within cyanobacterial mats can account for the origin of anammox bacteria and ancient nitrogen loss.Nature ecology & evolution · 2026Article
- Enrichment and characterization of a haloalkaline-tolerant anammox bacterium.The ISME journal · 2026Article
- Origin and Evolution of Key Enzymes in the Anammox Pathway Revisited.Genome biology and evolution · 2026Article
- Planktonic anammox bacteria toward a better understanding of ecophysiological traits and harnessing the untapped potential as a bioresource.Bioprocess and biosystems engineering · 2026Review
- Nitric oxide-forming nitrite reductases in the anaerobic ammonium oxidizer Kuenenia stuttgartiensis.FEBS open bio · 2025Article
- Nitrate-Nitrite Interplay in the Nitrogen Biocycle.Molecules (Basel, Switzerland) · 2025Review
- The mechanism and prospect of exogenous promoters enhancement Anammox at low temperatures: a review.World journal of microbiology & biotechnology · 2025Review
- Reversible Interconversion of Nitrate and Nitrite Catalyzed by Periplasmic Nitrate Reductase fromJournal of the American Chemical Society · 2025Article
- Influence of High Temperature and Ammonia and Nitrite Accumulation on the Physiological, Structural, and Genetic Aspects of the Biology of Largemouth Bass (Antioxidants (Basel, Switzerland) · 2025Article
- A powerful but frequently overlooked role of thermodynamics in environmental microbiology: inspirations from anammox.Applied and environmental microbiology · 2025Review
- Oxygen isotope fractionation during anaerobic ammonium oxidation by the marine representative Candidatus Scalindua sp.The ISME journal · 2025Article
- Magnesium application partially reversed the negative effects of mulching on rhizosphere nitrogen cycling in aFrontiers in plant science · 2025Article
- Fractionating proteins with nitrite-reducing activity in "Frontiers in microbiology · 2025Article
- Article
- Nitrite-oxidizing bacteria adapted to low-oxygen conditions dominate nitrite oxidation in marine oxygen minimum zones.The ISME journal · 2024Article
- Implications for nitrogen and sulphur cycles: phylogeny and niche-range ofISME communications · 2024Article
- Bringing Nitric Oxide to the Molybdenum World-A Personal Perspective.Molecules (Basel, Switzerland) · 2023Review
- Partitioning of the denitrification pathway and other nitrite metabolisms within global oxygen deficient zones.ISME communications · 2023Article
- Greater than the sum of parts: Mechanisms of metabolic regulation by enzyme filaments.Current opinion in structural biology · 2023Review
- Novel and unusual genes for nitrogen and metal cycling in Planctomycetota- and KSB1-affiliated metagenome-assembled genomes reconstructed from a marine subsea tunnel.FEMS microbiology letters · 2023Article
Corrections and comments
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Authors and funding
19 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nitrate is an abundant nutrient and electron acceptor throughout Earth's biosphere. Virtually all nitrate in nature is produced by the oxidation of nitrite by the nitrite oxidoreductase (NXR) multiprotein complex. NXR is a crucial enzyme in the global biological nitrogen cycle, and is found in nitrite-oxidizing bacteria (including comammox organisms), which generate the bulk of the nitrate in the environment, and in anaerobic ammonium-oxidizing (anammox) bacteria which produce half of the dinitrogen gas in our atmosphere. However, despite its central role in biology and decades of intense study, no structural information on NXR is available. Here, we present a structural and biochemical analysis of the NXR from the anammox bacterium Kuenenia stuttgartiensis, integrating X-ray crystallography, cryo-electron tomography, helical reconstruction cryo-electron microscopy, interaction and reconstitution studies and enzyme kinetics. We find that NXR catalyses both nitrite oxidation and nitrate reduction, and show that in the cell, NXR is arranged in tubules several hundred nanometres long. We reveal the tubule architecture and show that tubule formation is induced by a previously unidentified, haem-containing subunit, NXR-T. The results also reveal unexpected features in the active site of the enzyme, an unusual cofactor coordination in the protein's electron transport chain, and elucidate the electron transfer pathways within the complex.
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