ArticleThe Biochemical journal1987
Differential permeabilization of membranes by saponin treatment of isolated rat hepatocytes. Release of secretory proteins.
Article in The Biochemical journal, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 108 citations in OpenAlex.
- Saponin Chemistry Controls Anionic Lipid Tolerance and Divalent Metal Ion Responses in Magnetically Alignable Bicelles.bioRxiv : the preprint server for biology · 2026Article
- Stem cell-based strategies for intervertebral disc regeneration in degenerative microenvironments: challenges and solutions.Frontiers in cell and developmental biology · 2025Review
- Tracking fructose 1,6-bisphosphate dynamics in liver cancer cells using a fluorescent biosensor.iScience · 2024Article
- The ion channel TRPV5 regulates B-cell signaling and activation.Frontiers in immunology · 2024Article
- The fate of porcine sperm CRISP2 from the perinuclear theca before and after in vitro fertilization†.Biology of reproduction · 2022Article
- Chemical-Genetic Interactions ofMolecules (Basel, Switzerland) · 2021Article
- Dynamics of Cell Membrane Permeabilization by Saponins Using Terahertz Attenuated Total Reflection.Biophysical journal · 2020Article
- Membrane-specific spin trap, 5-dodecylcarbamoyl-5-N-dodecylacetamide-1-pyroline-N-oxide (diCOrganic & biomolecular chemistry · 2019Article
- Label-free self-referenced sensing of living cells by terahertz metamaterial-based reflection spectroscopy.Biomedical optics express · 2019Article
- Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts.Chemical reviews · 2018Review
- Review
- Comparative analysis of sphingomyelin synthase 1 gene expression at the transcriptional and translational levels in human tissues.Molecular and cellular biochemistry · 2015Article
- ATP-Binding Cassette Transporter Structure Changes Detected by Intramolecular Fluorescence Energy Transfer for High-Throughput Screening.Molecular pharmacology · 2015Article
- Transient partial permeabilization with saponin enables cellular barcoding prior to surface marker staining.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2014Article
- Cell- and virus-mediated regulation of the barrier-to-autointegration factor's phosphorylation state controls its DNA binding, dimerization, subcellular localization, and antipoxviral activity.Journal of virology · 2014Article
- Toxins in botanical dietary supplements: blue cohosh components disrupt cellular respiration and mitochondrial membrane potential.Journal of natural products · 2014Article
- Article
- Induction of highly curved structures in relation to membrane permeabilization and budding by the triterpenoid saponins, α- and δ-Hederin.The Journal of biological chemistry · 2013Article
- Monoclonal antibodies reveal multiple forms of expression of human microsomal epoxide hydrolase.Toxicology and applied pharmacology · 2012Article
- Coiled-coil domains enhance the membrane association of Salmonella type III effectors.Cellular microbiology · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Monolayer cultures of rat hepatocytes were treated with increasing concentrations of saponin (prepared from Gypsophila plants) for 30 min at 6 degrees C. Differential permeabilization of the intracellular membranes could be demonstrated: at 0.040 mg of saponin/ml the plasma membrane was permeabilized, as assessed by the release of 50% of the total cellular amount of lactate dehydrogenase, and at 0.20 mg/ml the endoplasmic reticulum was permeabilized, as measured by the release of 50% of pulse-35S-labelled albumin. The Golgi complex was permeabilized at an intermediate saponin concentration, as indicated by the release of homogeneously 35S-labelled albumin; about half the intracellular albumin is located in this organelle. At 1.0 up to 5.0 mg of saponin/ml 90-95% of the radioactively labelled albumin was released. Even at 5.0 mg/ml less than 10% of the membrane of the endoplasmic reticulum was solubilized, as judged by the degree of release of a membrane-bound enzyme specific for this organelle. These results demonstrate the usefulness of saponin as a tool for investigating the interior of different intracellular compartments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.