ArticleMethods in molecular biology (Clifton, N.J.)2021
Quantification of Staphylococcus aureus Biofilm Formation by Crystal Violet and Confocal Microscopy.
Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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Who cites it
30 citing papers in PubMed.
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- A Peptide Fraction from Brazilian Milk-Based Kefir Exhibits Antimicrobial and Antibiofilm Activities Against Clinically Relevant Bacteria.Probiotics and antimicrobial proteins · 2026Article
- Polydopamine-Antibiotic Composite Coating for Antibiofilm Applications.Macromolecular bioscience · 2026Article
- In vitro evaluation of antimicrobial activity of andrographolide against mixed biofilms of Candida albicans and Staphylococcus aureus.BMC complementary medicine and therapies · 2026Article
- Clinical severity-dependent virulence ofFrontiers in cellular and infection microbiology · 2026Article
- Systematic analysis of the type VII secretion system inFrontiers in microbiology · 2026Article
- Article
- A simple silicone elastomer colonization model highlights complexities ofJournal of medical microbiology · 2025Article
- Povidone-Iodine and Hydrogen Peroxide Combination Improves the Anti-Biofilm Activity of the Individual Agents onInternational journal of molecular sciences · 2025Article
- Antibiofilm activity of Plumbagin against Staphylococcus aureus.Scientific reports · 2025Article
- Heme alters biofilm formation inMicrobiology spectrum · 2025Article
- Epicatechin gallate and its analogues interact with sortase A and β-lactamase to suppressFrontiers in cellular and infection microbiology · 2025Article
- Biofilm-mediated resistance to berberine inFrontiers in cellular and infection microbiology · 2025Article
- Preparation of hafnium nitride-coated titanium implants by magnetron sputtering technology and evaluation of their antibacterial properties and biocompatibility.Open life sciences · 2025Article
- Phage Therapy: A Promising Treatment Strategy against Infections Caused by Multidrug-resistantCurrent pharmaceutical design · 2025Review
- GlmS plays a key role in the virulence factor expression and biofilm formation ability ofVirulence · 2024Article
- The effect of attachment systems and denture cleaning methods on microbial biomass and composition in implant-supported overdentures: an experimental study.International journal of implant dentistry · 2024Article
- Clinically relevant mutations in regulatory regions of metabolic genes facilitate early adaptation to ciprofloxacin in Escherichia coli.Nucleic acids research · 2024Article
- Repeated Exposure of Vancomycin to Vancomycin-Susceptible Staphylococcus aureus (VSSA) Parent Emerged VISA and VRSA Strains with Enhanced Virulence Potentials.Journal of microbiology (Seoul, Korea) · 2024Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most Staphylococcus aureus strains can grow as a multicellular biofilm, a phenotype of utmost importance to clinical infections such as endocarditis, osteomyelitis, and implanted medical device infection. As biofilms are inherently more tolerant to the host immune system and antibiotics, understanding the S. aureus genes and regulatory circuits that contribute to biofilm development is an active and on-going field of research. This chapter details a high-throughput and standardized way to grow S. aureus biofilms using a classical microtiter plate assay. Biofilms can be quantified using crystal violet or by confocal microscopy imaging and COMSTAT analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.